Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1252375..1252994 Replicon chromosome
Accession NZ_CP077809
Organism Klebsiella pneumoniae strain FF923

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KU666_RS05970 Protein ID WP_002892050.1
Coordinates 1252375..1252593 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KU666_RS05975 Protein ID WP_002892066.1
Coordinates 1252620..1252994 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU666_RS05935 (1248423) 1248423..1248686 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
KU666_RS05940 (1248686) 1248686..1248826 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KU666_RS05945 (1248823) 1248823..1249521 - 699 WP_002892021.1 GNAT family protein -
KU666_RS05950 (1249622) 1249622..1251072 + 1451 Protein_1140 PLP-dependent aminotransferase family protein -
KU666_RS05955 (1251047) 1251047..1251517 - 471 WP_032431532.1 YlaC family protein -
KU666_RS05960 (1251538) 1251538..1251678 + 141 WP_004147370.1 hypothetical protein -
KU666_RS05965 (1251650) 1251650..1252216 - 567 WP_002892030.1 maltose O-acetyltransferase -
KU666_RS05970 (1252375) 1252375..1252593 - 219 WP_002892050.1 HHA domain-containing protein Toxin
KU666_RS05975 (1252620) 1252620..1252994 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KU666_RS05980 (1253480) 1253480..1256626 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KU666_RS05985 (1256649) 1256649..1257842 - 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T207877 WP_002892050.1 NZ_CP077809:c1252593-1252375 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T207877 NZ_CP103530:c130996-130580 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT207877 WP_002892066.1 NZ_CP077809:c1252994-1252620 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT207877 NZ_CP103530:c131223-130993 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References