Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4815740..4816359 Replicon chromosome
Accession NZ_CP077805
Organism Klebsiella variicola strain FF907

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KU664_RS23440 Protein ID WP_002892050.1
Coordinates 4816141..4816359 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag KU664_RS23435 Protein ID WP_008805436.1
Coordinates 4815740..4816114 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU664_RS23425 (KU664_23355) 4810895..4812088 + 1194 WP_012542667.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KU664_RS23430 (KU664_23360) 4812111..4815257 + 3147 WP_008805437.1 multidrug efflux RND transporter permease subunit AcrB -
KU664_RS23435 (KU664_23365) 4815740..4816114 + 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
KU664_RS23440 (KU664_23370) 4816141..4816359 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KU664_RS23445 (KU664_23375) 4816518..4817084 + 567 WP_008805435.1 maltose O-acetyltransferase -
KU664_RS23450 (KU664_23380) 4817056..4817184 - 129 Protein_4578 hypothetical protein -
KU664_RS23455 (KU664_23385) 4817221..4817690 + 470 Protein_4579 YlaC family protein -
KU664_RS23460 (KU664_23390) 4817659..4819116 - 1458 WP_008805433.1 PLP-dependent aminotransferase family protein -
KU664_RS23465 (KU664_23395) 4819217..4819915 + 699 WP_064323876.1 GNAT family protein -
KU664_RS23470 (KU664_23400) 4819912..4820052 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KU664_RS23475 (KU664_23405) 4820052..4820315 - 264 WP_008805431.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T207852 WP_002892050.1 NZ_CP077805:4816141-4816359 [Klebsiella variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T207852 NZ_CP103524:19011-19358 [Escherichia coli]
ATGTGGACGGTACTTTTTAGCCAGCGATTTGACGACTGGCTAAATGAGCAAGAAGACGCTCTTCAGGAAAAGGTTTTGGC
TGATTTGAAAAATCTACAGGTTTACGGGCCTGAGTTACCCAGGCCATATGCCGATACCGTAAAAGGTTCTCGGTACAAAA
ATATGAAAGAGCTTCGCGTTCAGTTTTCTGGCCGTCCTATAAGAGCCTTTTATGCGTTCGATCCGATTCGCCGGGCTATC
GTTCTTTGTGCAGGAGACAAAAGTAACGATAAGCGGTTTTATAAAAAACTGGTGCGTATAGCTGAGGATGAGTTTGCAGC
ACATCTGAACACACTGGAGAGCAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT207852 WP_008805436.1 NZ_CP077805:4815740-4816114 [Klebsiella variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT207852 NZ_CP103524:19358-19657 [Escherichia coli]
ATGAGAACATTAGATGAGGTGATTGCCAGCCGTTCACCGGAAAGCCAGGCGCGAATTAAAGAAATGGCAGATGAGATGAT
TCTTGAGGTCGGCTTGCAGATGATGCGTGAAGAACTCCAGTTATCACAAAAACAGGTTGCTGAGGCGATGGGTATTAGTC
AGCCAGCAGTAACAAAGTTGGAGCAGCGCGGAAATGATTTAAAGCTGGCGACATTAAAGCGTTACGTTGAAGCTATGGGA
GGCAAATTAAGCCTGGATGTTGAGCTTCCCACAGGAAAACGAATAGCGTTTAACATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References