Detailed information of TA system TA207836

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 977878..978464
• Replicon: chromosome
• Accession: NZ_CP077805
• Organism: Klebsiella variicola strain FF907

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: KU664_RS04550
• Protein ID: WP_002920800.1
• Coordinates: 978096..978464 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: KU664_RS04545
• Protein ID: WP_023298302.1
• Coordinates: 977878..978099 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU664_RS04525 (KU664_04505)	974027..974953	+	927	WP_012540389.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
KU664_RS04530 (KU664_04510)	974950..976227	+	1278	WP_008806971.1	branched chain amino acid ABC transporter permease LivM	-
KU664_RS04535 (KU664_04515)	976224..976991	+	768	WP_008806972.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
KU664_RS04540 (KU664_04520)	976993..977706	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
KU664_RS04545 (KU664_04525)	977878..978099	+	222	WP_023298302.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KU664_RS04550 (KU664_04530)	978096..978464	+	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
KU664_RS04555 (KU664_04535)	978756..980072	+	1317	WP_087653350.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
KU664_RS04560 (KU664_04540)	980179..981066	+	888	WP_012967120.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
KU664_RS04565 (KU664_04545)	981063..981908	+	846	WP_008806976.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
KU664_RS04570 (KU664_04550)	981910..982980	+	1071	WP_008806977.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	972856..983717	10861

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T207836 WP_002920800.1 NZ_CP077805:978096-978464 [Klebsiella variicola]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T207836 NZ_CP103521:3029279-3029773 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8123.14 Da
• Isoelectric Point: 4.7769

>AT207836 WP_023298302.1 NZ_CP077805:977878-978099 [Klebsiella variicola]
MKTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT207836 NZ_CP103521:3028989-3029282 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAACCAGGCGCGAATCTTTAAATTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1