Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4078038..4078657 Replicon chromosome
Accession NZ_CP077779
Organism Klebsiella pneumoniae strain CDI231

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KUM55_RS19955 Protein ID WP_002892050.1
Coordinates 4078439..4078657 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KUM55_RS19950 Protein ID WP_002892066.1
Coordinates 4078038..4078412 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KUM55_RS19940 (KUM55_19945) 4073190..4074383 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KUM55_RS19945 (KUM55_19950) 4074406..4077552 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KUM55_RS19950 (KUM55_19955) 4078038..4078412 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KUM55_RS19955 (KUM55_19960) 4078439..4078657 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KUM55_RS19960 (KUM55_19965) 4078816..4079382 + 567 WP_002892030.1 maltose O-acetyltransferase -
KUM55_RS19965 (KUM55_19970) 4079354..4079494 - 141 WP_004147370.1 hypothetical protein -
KUM55_RS19970 (KUM55_19975) 4079515..4079985 + 471 WP_002892026.1 YlaC family protein -
KUM55_RS19975 (KUM55_19980) 4079960..4081411 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
KUM55_RS19980 (KUM55_19985) 4081512..4082210 + 699 WP_004177238.1 GNAT family protein -
KUM55_RS19985 (KUM55_19990) 4082207..4082347 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KUM55_RS19990 (KUM55_19995) 4082347..4082610 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T207812 WP_002892050.1 NZ_CP077779:4078439-4078657 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T207812 NZ_CP103515:c3997926-3997639 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT207812 WP_002892066.1 NZ_CP077779:4078038-4078412 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT207812 NZ_CP103515:c3997652-3997350 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References