Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 3232601..3233232 Replicon chromosome
Accession NZ_CP077779
Organism Klebsiella pneumoniae strain CDI231

Toxin (Protein)


Gene name hicA Uniprot ID A0A1Q8YTV3
Locus tag KUM55_RS15925 Protein ID WP_012542177.1
Coordinates 3233056..3233232 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A3Q9U6R4
Locus tag KUM55_RS15920 Protein ID WP_017898984.1
Coordinates 3232601..3233008 (-) Length 136 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KUM55_RS15885 (KUM55_15890) 3227965..3228399 - 435 WP_012542168.1 phage terminase small subunit P27 family -
KUM55_RS15890 (KUM55_15895) 3228647..3229078 - 432 WP_023279521.1 hypothetical protein -
KUM55_RS15895 (KUM55_15900) 3229075..3229392 - 318 WP_023279522.1 hypothetical protein -
KUM55_RS15900 (KUM55_15905) 3229344..3229706 - 363 WP_014228901.1 HNH endonuclease -
KUM55_RS15905 (KUM55_15910) 3230831..3231181 - 351 WP_017898986.1 hypothetical protein -
KUM55_RS15910 (KUM55_15915) 3231178..3231675 - 498 WP_023279523.1 lysozyme -
KUM55_RS15915 (KUM55_15920) 3231675..3231890 - 216 WP_017880269.1 class II holin family protein -
KUM55_RS15920 (KUM55_15925) 3232601..3233008 - 408 WP_017898984.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
KUM55_RS15925 (KUM55_15930) 3233056..3233232 - 177 WP_012542177.1 type II toxin-antitoxin system HicA family toxin Toxin
KUM55_RS15930 (KUM55_15935) 3233596..3235380 + 1785 WP_032415155.1 ATP-binding protein -
KUM55_RS15935 (KUM55_15940) 3235400..3236434 + 1035 WP_071600377.1 DNA cytosine methyltransferase -
KUM55_RS15940 (KUM55_15945) 3236459..3236800 - 342 WP_017898982.1 antiterminator Q family protein -
KUM55_RS15945 (KUM55_15950) 3236813..3237844 - 1032 WP_071058942.1 DUF968 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3199201..3254733 55532


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-61)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6632.83 Da        Isoelectric Point: 11.0174

>T207811 WP_012542177.1 NZ_CP077779:c3233232-3233056 [Klebsiella pneumoniae]
VKQSELRRWLAAQGAEFKDGTNHLKIYLNGKQTVMPRHPGKEIPEPLRKAILKQLGIK

Download         Length: 177 bp

>T207811 NZ_CP103515:3661845-3662123 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 136 a.a.        Molecular weight: 14959.96 Da        Isoelectric Point: 4.4277

>AT207811 WP_017898984.1 NZ_CP077779:c3233008-3232601 [Klebsiella pneumoniae]
MRYPVIFEHDETGWAVFFPDIPEAMTGGETREEALEMAQDALVTAFDFYFDDRREIPAPSAEGDAFVEVPASVAAKVLLL
NRLVSTNTSNADLARLINTRPQEVQRIVSLGHSTKIDTIQKALSALGQKMEIVVH

Download         Length: 408 bp

>AT207811 NZ_CP103515:3661582-3661842 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q8YTV3

Antitoxin

Source ID Structure
AlphaFold DB A0A3Q9U6R4

References