Detailed information of TA system TA207280

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1323214..1324051
• Replicon: chromosome
• Accession: NZ_CP077649
• Organism: Escherichia coli strain ST865

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: KTN07_RS06465
• Protein ID: WP_000227784.1
• Coordinates: 1323509..1324051 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: KTN07_RS06460
• Protein ID: WP_001297137.1
• Coordinates: 1323214..1323525 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KTN07_RS06435 (1318234)	1318234..1319181	+	948	WP_217609421.1	cytochrome o ubiquinol oxidase subunit II	-
KTN07_RS06440 (1319203)	1319203..1321194	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
KTN07_RS06445 (1321184)	1321184..1321798	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
KTN07_RS06450 (1321798)	1321798..1322127	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
KTN07_RS06455 (1322139)	1322139..1323029	+	891	WP_135301382.1	heme o synthase	-
KTN07_RS06460 (1323214)	1323214..1323525	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
KTN07_RS06465 (1323509)	1323509..1324051	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
KTN07_RS06470 (1324107)	1324107..1325042	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
KTN07_RS06475 (1325450)	1325450..1326814	+	1365	WP_001000978.1	MFS transporter	-
KTN07_RS06480 (1326942)	1326942..1327433	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
KTN07_RS06485 (1327601)	1327601..1328512	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T207280 WP_000227784.1 NZ_CP077649:1323509-1324051 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T207280 NZ_CP077649:1323509-1324051 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT207280 WP_001297137.1 NZ_CP077649:1323214-1323525 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT207280 NZ_CP077649:1323214-1323525 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3