Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4812782..4813413 Replicon chromosome
Accession NZ_CP077293
Organism Klebsiella aerogenes strain FDAARGOS 1442

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H3FL31
Locus tag I6L72_RS22830 Protein ID WP_015703741.1
Coordinates 4813138..4813413 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0H3FNK6
Locus tag I6L72_RS22825 Protein ID WP_015703740.1
Coordinates 4812782..4813141 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L72_RS22800 (I6L72_22800) 4809039..4809983 + 945 WP_015703735.1 nickel ABC transporter permease subunit NikB -
I6L72_RS22805 (I6L72_22805) 4809980..4810813 + 834 WP_015703736.1 nickel ABC transporter permease subunit NikC -
I6L72_RS22810 (I6L72_22810) 4810813..4811577 + 765 WP_015703737.1 nickel import ATP-binding protein NikD -
I6L72_RS22815 (I6L72_22815) 4811574..4812365 + 792 WP_015703738.1 nickel import ATP-binding protein NikE -
I6L72_RS22820 (I6L72_22820) 4812353..4812754 + 402 WP_015703739.1 nickel-responsive transcriptional regulator NikR -
I6L72_RS22825 (I6L72_22825) 4812782..4813141 - 360 WP_015703740.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
I6L72_RS22830 (I6L72_22830) 4813138..4813413 - 276 WP_015703741.1 type II toxin-antitoxin system HicA family toxin Toxin
I6L72_RS22835 (I6L72_22835) 4813810..4814823 + 1014 WP_015369274.1 magnesium transporter -
I6L72_RS22840 (I6L72_22840) 4814864..4816057 - 1194 WP_015703742.1 NAD(P)/FAD-dependent oxidoreductase -
I6L72_RS22845 (I6L72_22845) 4816288..4817784 + 1497 WP_015369272.1 inorganic phosphate transporter PitA -
I6L72_RS22850 (I6L72_22850) 4817849..4818184 - 336 WP_015703743.1 universal stress protein UspB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10258.89 Da        Isoelectric Point: 10.4888

>T206994 WP_015703741.1 NZ_CP077293:c4813413-4813138 [Klebsiella aerogenes]
MEQQVLSLRKKQRNTLEQLFKTPVPQGIKWADIESLIKALGGEIKEGRGSRCKFLLNHSIASFHRPHPSPDTDKGAVESV
RDWLITIGVKP

Download         Length: 276 bp

>T206994 NZ_CP102836:c4843152-4842868 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13305.02 Da        Isoelectric Point: 4.3472

>AT206994 WP_015703740.1 NZ_CP077293:c4813141-4812782 [Klebsiella aerogenes]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQQEGEISLQEYLADCREAGIEPYAQPEKLKT
FTLRYPESFGERLSSAAAEEQVSVNTWILETLNERLKQA

Download         Length: 360 bp

>AT206994 NZ_CP102836:c4843384-4843142 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3FL31

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3FNK6

References