Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 25929..26788 Replicon plasmid unnamed2
Accession NZ_CP077213
Organism Enterobacter cloacae strain FDAARGOS 1431

Toxin (Protein)


Gene name yhaV Uniprot ID A0A8B3V8K3
Locus tag I6L61_RS27110 Protein ID WP_013087279.1
Coordinates 25929..26423 (-) Length 165 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A8B3VF40
Locus tag I6L61_RS27115 Protein ID WP_013087280.1
Coordinates 26444..26788 (-) Length 115 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L61_RS27080 (I6L61_27080) 21774..22043 + 270 WP_237707076.1 hypothetical protein -
I6L61_RS27085 (I6L61_27085) 22435..23193 - 759 WP_013087274.1 hypothetical protein -
I6L61_RS27090 (I6L61_27090) 23356..24204 - 849 WP_013087275.1 DUF4942 domain-containing protein -
I6L61_RS27095 (I6L61_27095) 24394..25074 - 681 WP_013087276.1 hypothetical protein -
I6L61_RS27100 (I6L61_27100) 25171..25419 - 249 WP_044160311.1 hypothetical protein -
I6L61_RS27105 (I6L61_27105) 25733..25927 + 195 WP_139348812.1 hypothetical protein -
I6L61_RS27110 (I6L61_27110) 25929..26423 - 495 WP_013087279.1 type II toxin-antitoxin system YhaV family toxin Toxin
I6L61_RS27115 (I6L61_27115) 26444..26788 - 345 WP_013087280.1 type II toxin-antitoxin system PrlF family antitoxin Antitoxin
I6L61_RS27120 (I6L61_27120) 27110..27328 - 219 WP_013087281.1 type II toxin-antitoxin system VapB family antitoxin -
I6L61_RS27125 (I6L61_27125) 27568..27798 - 231 WP_013087283.1 hypothetical protein -
I6L61_RS27130 (I6L61_27130) 27789..28052 - 264 WP_013087284.1 DUF1380 family protein -
I6L61_RS27135 (I6L61_27135) 28159..28440 - 282 WP_044160304.1 hypothetical protein -
I6L61_RS27140 (I6L61_27140) 28588..29106 - 519 WP_013087286.1 hypothetical protein -
I6L61_RS27145 (I6L61_27145) 29096..29470 - 375 WP_044160300.1 hypothetical protein -
I6L61_RS27150 (I6L61_27150) 29482..29829 - 348 WP_079972020.1 hypothetical protein -
I6L61_RS27155 (I6L61_27155) 29858..30289 - 432 WP_013087289.1 antirestriction protein -
I6L61_RS27160 (I6L61_27160) 30604..30843 + 240 WP_237707077.1 hypothetical protein -
I6L61_RS27165 (I6L61_27165) 31106..31543 - 438 WP_013087292.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..86314 86314


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(11-147)

Antitoxin

PrlF antitoxin

(16-114)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 19174.06 Da        Isoelectric Point: 8.6417

>T206814 WP_013087279.1 NZ_CP077213:c26423-25929 [Enterobacter cloacae]
MEYLELNGWKICFHKCFLDQIAELAQKVAELKAAKPNEYHKKKETKLLIAIERVIEEWIASDPLNPQYRQGDTLGDEYKH
WFRVKFLQQFRLFYRCSQEHKTIIIGWVNDFDTLRAYGSKTDAYKVFAEMLKAGTPPDDWNTLLNAAKKATATNTIPGFL
TRQP

Download         Length: 495 bp

>T206814 NZ_CP102692:c2590557-2589235 [Klebsiella pneumoniae]
ATGGCGACACTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGACGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGGCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
AAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCGCCACTCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin


Download         Length: 115 a.a.        Molecular weight: 12515.27 Da        Isoelectric Point: 4.4335

>AT206814 WP_013087280.1 NZ_CP077213:c26788-26444 [Enterobacter cloacae]
MATQMLNSADVSLRAESRLTERSQTTIPAAIRDALHLKPGESIHYTLLAGGKVIMSKKEEEQDDPVVPQFLAFLENDMKK
NPQNIAPVPALFWESIKALTAGVEVDLDAPLTDD

Download         Length: 345 bp

>AT206814 NZ_CP102692:c2590823-2590557 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGAGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3V8K3

Antitoxin

Source ID Structure
AlphaFold DB A0A8B3VF40

References