Detailed information of TA system TA206454

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4318848..4319452
• Replicon: chromosome
• Accession: NZ_CP076723
• Organism: Geomonas oryzisoli strain RG10

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KP004_RS18750
• Protein ID: WP_199390331.1
• Coordinates: 4319171..4319452 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: KP004_RS18745
• Protein ID: WP_199390330.1
• Coordinates: 4318848..4319162 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KP004_RS18720 (KP004_18720)	4314157..4314894	-	738	WP_216799918.1	LytTR family DNA-binding domain-containing protein	-
KP004_RS18725 (KP004_18725)	4314898..4315293	-	396	WP_216799919.1	type II toxin-antitoxin system VapC family toxin	-
KP004_RS18730 (KP004_18730)	4315280..4315519	-	240	WP_216799920.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
KP004_RS18735 (KP004_18735)	4315830..4318142	+	2313	WP_216799921.1	hypothetical protein	-
KP004_RS18740 (KP004_18740)	4318326..4318811	-	486	WP_216799922.1	VOC family protein	-
KP004_RS18745 (KP004_18745)	4318848..4319162	-	315	WP_199390330.1	HigA family addiction module antitoxin	Antitoxin
KP004_RS18750 (KP004_18750)	4319171..4319452	-	282	WP_199390331.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KP004_RS18755 (KP004_18755)	4319602..4320867	-	1266	WP_216799923.1	hypothetical protein	-
KP004_RS18760 (KP004_18760)	4321360..4321911	-	552	WP_216799924.1	chalcone isomerase family protein	-
KP004_RS18765 (KP004_18765)	4322204..4322497	+	294	WP_216799925.1	hypothetical protein	-
KP004_RS18770 (KP004_18770)	4322506..4322808	+	303	WP_216799926.1	hypothetical protein	-
KP004_RS18775 (KP004_18775)	4323085..4323261	+	177	WP_216799927.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4318848..4340107	21259

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (28-74)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10859.38 Da
• Isoelectric Point: 8.9224

>T206454 WP_199390331.1 NZ_CP076723:c4319452-4319171 [Geomonas oryzisoli]
VIKTFADKDTKQLFLTGKAKRLPPDLFGRARRRLEYIDLASVLEDLKVPPSNRLHPLHGSRYGQHAISINDQWRICFVWH
NGDAYEVEITDYH

Nucleotide

• Length: 282 bp

>T206454 NZ_CP102434:1352466-1352735 [Vibrio parahaemolyticus]
ATGAGTAGTAGTCAACGTTTATTATCGTGGACTGATGATGCTTGGGATGACTACCTGTATTGGCAAACTCAAGACAAGAA
AACACTCAAGCGCATCAATAAACTCATCAATGATGTTAAGCGCTCTCCATTTGAGGGCATTGGTAAACCAGAGCCGTTAA
AAGAGAACTTATCTGGTTTTTGGTCTCGTCGTATTGATGATACTAATAGGCTTGTTTACGCAGTCGATGATCAAGCGATA
ACGATAATTTCATGTCGTTACCACTACTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11766.55 Da
• Isoelectric Point: 10.4384

>AT206454 WP_199390330.1 NZ_CP076723:c4319162-4318848 [Geomonas oryzisoli]
MFKSDRDKREIPPTHPGAMLREDFMPDYGLTAATLAGTLGVSRQTINELLRERRALSPGMALRLSRLFGNTPEFWLNAQR
AVDLWQAEKDLAKELARIRSLQAA

Nucleotide

• Length: 315 bp

>AT206454 NZ_CP102434:1352219-1352473 [Vibrio parahaemolyticus]
ATGAGAATCGTATCTTTTACTGAAGCTAGAAATGGTCTCAAAGCTGTTTTAGACGGTGTAGTTAATGATGCTGATACAAC
AGTTATTACACGCCGTGATTCTGAGGATGCAGTGGTTATGTCTTTAGACTACTACAATAGCCTTATGGAGACTGTTCATT
TACTACGCTCTCCTCAAAACGTTGAACACCTAAACCGTTCGATAGCACAGTACCGCGCTGGTAAAACAACAGCACGAGAG
TTAATTGATGAGTAG