Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | tacAT/DUF1778(antitoxin) |
| Location | 55251..56026 | Replicon | plasmid unnamed |
| Accession | NZ_CP076705 | ||
| Organism | Escherichia coli O8:H28 strain BMH-17-0027 | ||
Toxin (Protein)
| Gene name | tacT | Uniprot ID | A0A8D9PPA8 |
| Locus tag | KQ221_RS23375 | Protein ID | WP_000405244.1 |
| Coordinates | 55251..55733 (-) | Length | 161 a.a. |
Antitoxin (Protein)
| Gene name | tacA | Uniprot ID | - |
| Locus tag | KQ221_RS23380 | Protein ID | WP_001346256.1 |
| Coordinates | 55724..56026 (-) | Length | 101 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KQ221_RS23345 (50563) | 50563..50859 | + | 297 | WP_153188706.1 | hypothetical protein | - |
| KQ221_RS23350 (50859) | 50859..52046 | + | 1188 | WP_097446502.1 | IS91 family transposase | - |
| KQ221_RS23355 (52314) | 52314..52553 | - | 240 | WP_097446501.1 | helix-turn-helix domain-containing protein | - |
| KQ221_RS23360 (53527) | 53527..54384 | - | 858 | WP_000130957.1 | incFII family plasmid replication initiator RepA | - |
| KQ221_RS23935 (54377) | 54377..54424 | - | 48 | WP_235246954.1 | hypothetical protein | - |
| KQ221_RS23940 (54415) | 54415..54648 | + | 234 | WP_226058127.1 | replication protein RepA | - |
| KQ221_RS23370 (54684) | 54684..54941 | - | 258 | WP_097446499.1 | replication regulatory protein RepA | - |
| KQ221_RS23375 (55251) | 55251..55733 | - | 483 | WP_000405244.1 | GNAT family N-acetyltransferase | Toxin |
| KQ221_RS23380 (55724) | 55724..56026 | - | 303 | WP_001346256.1 | DUF1778 domain-containing protein | Antitoxin |
| KQ221_RS23385 (56348) | 56348..56497 | - | 150 | WP_001312851.1 | Hok/Gef family protein | - |
| - (56542) | 56542..56601 | + | 60 | NuclAT_1 | - | - |
| - (56542) | 56542..56601 | + | 60 | NuclAT_1 | - | - |
| - (56542) | 56542..56601 | + | 60 | NuclAT_1 | - | - |
| - (56542) | 56542..56601 | + | 60 | NuclAT_1 | - | - |
| KQ221_RS23945 (56863) | 56863..56931 | - | 69 | Protein_50 | endonuclease | - |
| KQ221_RS23395 (57177) | 57177..57389 | - | 213 | WP_032289176.1 | hypothetical protein | - |
| KQ221_RS23400 (57519) | 57519..58079 | - | 561 | WP_097446497.1 | fertility inhibition protein FinO | - |
| KQ221_RS23405 (58134) | 58134..58880 | - | 747 | WP_097446496.1 | conjugal transfer pilus acetylase TraX | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | - | hlyD / hlyB / hlyA / hlyC | 1..117710 | 117710 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 161 a.a. Molecular weight: 17659.41 Da Isoelectric Point: 7.1036
>T206370 WP_000405244.1 NZ_CP076705:c55733-55251 [Escherichia coli O8:H28]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLHGRAMKNQMLNASRTFVICLEGTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
MEINVTAPALLTDEHILQPFDCGNEVLSNWLHGRAMKNQMLNASRTFVICLEGTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
Download Length: 483 bp
>T206370 NZ_CP102378:3287973-3288437 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA
Antitoxin
Download Length: 101 a.a. Molecular weight: 11602.19 Da Isoelectric Point: 4.4569
>AT206370 WP_001346256.1 NZ_CP076705:c56026-55724 [Escherichia coli O8:H28]
MEVVMPTTASIRKTPRENQINIRATDEERAVIDYAASLVNKNRTDFIIELAYQEAKNIILDQRLFVLDDECYDSFITQLE
APVQNAEGRERLMAVKPEWK
MEVVMPTTASIRKTPRENQINIRATDEERAVIDYAASLVNKNRTDFIIELAYQEAKNIILDQRLFVLDDECYDSFITQLE
APVQNAEGRERLMAVKPEWK
Download Length: 303 bp
>AT206370 NZ_CP102378:3287638-3287973 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|