Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4817273..4817868 Replicon chromosome
Accession NZ_CP076697
Organism Escherichia coli strain S10EC

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag KQ259_RS23330 Protein ID WP_000239579.1
Coordinates 4817518..4817868 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag KQ259_RS23325 Protein ID WP_001223208.1
Coordinates 4817273..4817524 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ259_RS23315 (4812938) 4812938..4816717 + 3780 WP_000060945.1 autotransporter assembly complex protein TamB -
KQ259_RS23320 (4816720) 4816720..4817061 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
KQ259_RS23325 (4817273) 4817273..4817524 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
KQ259_RS23330 (4817518) 4817518..4817868 + 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
KQ259_RS23335 (4817948) 4817948..4818478 - 531 WP_000055075.1 inorganic diphosphatase -
KQ259_RS23340 (4818788) 4818788..4819744 + 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
KQ259_RS23345 (4819884) 4819884..4821386 + 1503 WP_000205813.1 sugar ABC transporter ATP-binding protein -
KQ259_RS23350 (4821400) 4821400..4822422 + 1023 WP_001296689.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T206334 WP_000239579.1 NZ_CP076697:4817518-4817868 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T206334 NZ_CP102359:2197591-2197845 [Streptomyces sp. B146]
GTGAGGGTCACTTTCACGTCCCACGGCTGGGAGGACTACGTCCACTGGGCCGAGAGCGACCGGAAGGTGACCAAGCGGAT
CAACAGACTGATCGACGACATCACCCGTGACCCGTTCAAGGGCATCGGGAAGCCGGAGCCGCTCAAGGGCGACCTGTCGG
GCTACTGGTCACGGCGCATCGACGACACGCACCGGCTCGTGTACAAGCCCGCCGACGGCGTACTGGTCATCGTGCAGGCC
CGCTATCACTACTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT206334 WP_001223208.1 NZ_CP076697:4817273-4817524 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT206334 NZ_CP102359:2197331-2197594 [Streptomyces sp. B146]
ATGCCCATCACCGCCAGCGAAGCCCGTCAGAACCTGTTCCCGCTGATAGAGCAGGTCAACGAGGACCATGCCCCGGTACA
CATCACCTCCCGCAAGGGAAACGCCGTACTCATGTCCGAGGAGGACTTCACGGCGTGGACGGAGACGGTGCACCTCCTGC
GCTCGCCCAAGAACGCCCGCCGTCTGCTCGACTCCATCGCGGAGGCCGAAGCGGGCGAAGCACGGCATCGCGAGCTGATC
GACCCGGACGCGGAGCGGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References