Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-VapI
Location 1443548..1444203 Replicon chromosome
Accession NC_002971
Organism Coxiella burnetii RSA 493

Toxin (Protein)


Gene name graT Uniprot ID Q83BL3
Locus tag CBU_1491 Protein ID NP_820474.2
Coordinates 1443865..1444203 (-) Length 113 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID Q83BL4
Locus tag CBU_1490 Protein ID NP_820473.2
Coordinates 1443548..1443859 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CBU_1485 1438826..1439503 - 678 NP_820468.2 cyclin protein -
CBU_1486 1439600..1439767 + 168 NP_820469.1 hypothetical protein -
CBU_1487 1439821..1441203 - 1383 NP_820470.2 cysteine--tRNA ligase -
CBU_1488 1441194..1442591 - 1398 NP_820471.2 glutamine--tRNA ligase -
CBU_1489 1442748..1443479 + 732 NP_820472.1 UDP-2,3-diacylglucosamine hydrolase -
CBU_1490 1443548..1443859 - 312 NP_820473.2 virulence-associated protein I Antitoxin
CBU_1491 1443865..1444203 - 339 NP_820474.2 AAA family ATPase Toxin
CBU_1492 1444562..1445707 + 1146 NP_820475.1 transposase -
CBU_1493 1446035..1447708 + 1674 NP_820476.2 hypothetical protein -
CBU_1494 1447882..1448637 - 756 NP_820477.2 pyridoxal phosphate biosynthetic protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1444562..1445707 1145


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(23-112)

Antitoxin

Cro/C1-type helix-turn-helix domain

(26-72)


Sequences

Toxin        


Download         Length: 113 a.a.        Molecular weight: 13409.66 Da        Isoelectric Point: 10.4588

>T20626 NP_820474.2 NC_002971:c1444203-1443865 [Coxiella burnetii RSA 493]
MPLTLYIMLDVTRKTVILEVIIKSFKDKYTKYLYKGVSVSKWQAIRKQAERRLQILDSVTSLDDLRSLPSNRFESLRGNR
KGQFSIRINKQWRICFKWINNEPTEVEIVDYH

Download         Length: 339 bp

>T20626 NC_002971:c1444203-1443865 [Coxiella burnetii RSA 493]
TTGCCTCTTACGCTGTATATTATGCTTGATGTAACGCGTAAAACTGTTATCCTTGAAGTGATAATAAAAAGTTTCAAGGA
CAAATACACTAAATATTTATATAAAGGTGTATCCGTTTCAAAATGGCAAGCCATACGAAAGCAGGCGGAAAGACGGCTTC
AGATTTTGGATTCAGTGACATCGTTAGATGATTTGAGAAGTTTGCCAAGCAATCGTTTTGAATCGTTGCGGGGGAATAGA
AAAGGACAATTTAGTATAAGGATTAATAAGCAATGGCGCATTTGTTTTAAGTGGATTAACAATGAGCCAACTGAAGTTGA
AATTGTGGATTATCATTAG

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11458.39 Da        Isoelectric Point: 10.1699

>AT20626 NP_820473.2 NC_002971:c1443859-1443548 [Coxiella burnetii RSA 493]
MKMAANRMRPIHPGEILAEELGFLDKMSANQLAKHLAIPTNRVTAILNGARSITADTALRLAKFFGTTPEFWLNLQDAYD
IKMALKKSGKKIEKEVTPYDQAA

Download         Length: 312 bp

>AT20626 NC_002971:c1443859-1443548 [Coxiella burnetii RSA 493]
ATGAAAATGGCAGCTAATAGAATGCGACCCATACATCCAGGTGAGATCCTTGCTGAAGAGTTGGGGTTTTTAGATAAGAT
GTCAGCTAATCAGTTAGCTAAACATTTAGCAATTCCGACCAACCGAGTGACCGCCATTCTCAATGGCGCGCGATCCATTA
CGGCGGATACCGCGCTACGATTGGCTAAGTTTTTTGGGACGACGCCGGAATTTTGGCTAAATCTCCAAGACGCCTATGAC
ATAAAAATGGCATTGAAAAAGTCCGGCAAAAAAATCGAAAAAGAAGTAACCCCTTACGACCAAGCCGCCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q83BL3

Antitoxin

Source ID Structure
PDB 3TRB
AlphaFold DB Q83BL4

References