Detailed information of TA system TA206161

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 54518..54943
• Replicon: plasmid unnamed
• Accession: NZ_CP076655
• Organism: Escherichia coli strain NC19

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KPE96_RS23450
• Protein ID: WP_227898123.1
• Coordinates: 54824..54943 (+)
• Length: 40 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	54518..54729 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPE96_RS23425 (50492)	50492..50989	+	498	WP_050427786.1	single-stranded DNA-binding protein	-
KPE96_RS23430 (51052)	51052..51285	+	234	WP_001209608.1	DUF905 domain-containing protein	-
KPE96_RS23435 (51344)	51344..53302	+	1959	Protein_49	ParB/RepB/Spo0J family partition protein	-
KPE96_RS23440 (53357)	53357..53791	+	435	WP_000845935.1	conjugation system SOS inhibitor PsiB	-
KPE96_RS23445 (53788)	53788..54507	+	720	WP_001276275.1	plasmid SOS inhibition protein A	-
- (54518)	54518..54729	+	212	NuclAT_0	-	Antitoxin
- (54518)	54518..54729	+	212	NuclAT_0	-	Antitoxin
- (54518)	54518..54729	+	212	NuclAT_0	-	Antitoxin
- (54518)	54518..54729	+	212	NuclAT_0	-	Antitoxin
KPE96_RS24220 (54763)	54763..54876	+	114	Protein_52	DUF5431 family protein	-
KPE96_RS23450 (54824)	54824..54943	+	120	WP_227898123.1	Hok/Gef family protein	Toxin
KPE96_RS24225 (55244)	55244..55565	-	322	Protein_54	hypothetical protein	-
KPE96_RS24230 (55567)	55567..55853	+	287	Protein_55	hypothetical protein	-
KPE96_RS23460 (55972)	55972..56793	+	822	WP_001234485.1	DUF932 domain-containing protein	-
KPE96_RS23465 (57089)	57089..57691	-	603	WP_023145258.1	transglycosylase SLT domain-containing protein	-
KPE96_RS23470 (58013)	58013..58396	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KPE96_RS23475 (58586)	58586..59272	+	687	WP_000332490.1	PAS domain-containing protein	-
KPE96_RS23480 (59366)	59366..59593	+	228	WP_001442099.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	sitABCD	iutA / iucD / iucC / iucB / iucA / vat / iroN / iroE / iroD / iroC / iroB	1..166302	166302

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-34)

Sequences

Toxin

Protein

• Length: 40 a.a.
• Molecular weight: 4423.19 Da
• Isoelectric Point: 8.2691

>T206161 WP_227898123.1 NZ_CP076655:54824-54943 [Escherichia coli]
VCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 120 bp

>T206161 NZ_CP102176:488398-488685 [Pseudomonas mediterranea]
ATGACCGAACAATTCACTCGTTGGGACTCTGCCGAGTACCTCAAGACCGAAGAGGACATGGCCAATTATCTGGATGCATG
CATGGAGGAGGCCGGAGACGATCCCGCATTCATCGCAAAAGCCCTGGGGACCATTGCACGAGCTCGCGGAATGAGCCAAG
TGGCCCGTGATGCAGGGTTGTCACGTGAGAATCTTTATCGTGCCTTGTCGGGGGAAGGCAATCCTGAATTTGGAACCATT
TTGAAGGTTGTCAGAGCGTTGGGGTTGAAGCTGCATGCCAGTACTTGA

Antitoxin

Nucleotide

• Length: 212 bp

>AT206161 NZ_CP076655:54518-54729 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTCTTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
AGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGC
AGAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Antitoxin