Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 3739633..3740245 Replicon chromosome
Accession NZ_CP076596
Organism Erwinia amylovora isolate CP20161301

Toxin (Protein)


Gene name hicA Uniprot ID D2TUP9
Locus tag KQH32_RS16960 Protein ID WP_012906750.1
Coordinates 3739633..3739812 (+) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID D4I356
Locus tag KQH32_RS16965 Protein ID WP_004157632.1
Coordinates 3739835..3740245 (+) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQH32_RS16940 (KQH32_16950) 3735191..3736141 + 951 WP_004157624.1 DUF1471 family protein YdgH -
KQH32_RS16945 (KQH32_16955) 3736349..3737740 + 1392 WP_004157625.1 amino acid permease -
KQH32_RS16950 (KQH32_16960) 3737744..3738208 - 465 WP_004157626.1 hypothetical protein -
KQH32_RS16955 (KQH32_16965) 3738385..3739113 + 729 WP_004157629.1 two-component system response regulator RstA -
KQH32_RS16960 (KQH32_16970) 3739633..3739812 + 180 WP_012906750.1 type II toxin-antitoxin system HicA family toxin Toxin
KQH32_RS16965 (KQH32_16975) 3739835..3740245 + 411 WP_004157632.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
KQH32_RS16970 (KQH32_16980) 3740301..3741302 + 1002 WP_004157633.1 acyltransferase -
KQH32_RS16975 (KQH32_16985) 3742173..3742385 - 213 WP_004157635.1 hypothetical protein -
KQH32_RS16980 (KQH32_16990) 3742643..3742816 - 174 WP_004157636.1 hypothetical protein -
KQH32_RS16985 (KQH32_16995) 3742911..3743084 - 174 WP_013036045.1 hypothetical protein -
KQH32_RS16990 (KQH32_17000) 3743095..3743367 - 273 WP_004157637.1 hypothetical protein -
KQH32_RS16995 (KQH32_17005) 3743997..3744587 + 591 WP_223386180.1 DapH/DapD/GlmU-related protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3733471..3744587 11116


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-126)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6644.85 Da        Isoelectric Point: 10.9132

>T206031 WP_012906750.1 NZ_CP076596:3739633-3739812 [Erwinia amylovora]
MDSRNAIAMIEADGWYLVRVKGSHHQFKHPTKKGLVTVKHPQKDIPLPTLKSIKKQAGL

Download         Length: 180 bp

>T206031 NZ_CP102077:837779-838189 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 14829.76 Da        Isoelectric Point: 4.3623

>AT206031 WP_004157632.1 NZ_CP076596:3739835-3740245 [Erwinia amylovora]
MLYPVAIDKGDSSFGVRVPDIPGCFSGGDNYQDAIESAREAIDAHIELLVEDGEAVPEATSVENWLADPDYVGVVWALVD
VDVTRLMGKAEKINVTLPSLLIRRIDQFVAAHPEYGSRSGFLSRVAADKVIGREKR

Download         Length: 411 bp

>AT206031 NZ_CP102077:837532-837798 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7D6XDK4

Antitoxin

Source ID Structure
AlphaFold DB A0A830ZVY0

References