Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2568719..2569385 Replicon chromosome
Accession NZ_CP076596
Organism Erwinia amylovora isolate CP20161301

Toxin (Protein)


Gene name cptA Uniprot ID A0A830ZTB8
Locus tag KQH32_RS11490 Protein ID WP_004161808.1
Coordinates 2568966..2569385 (+) Length 140 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID D4HWC2
Locus tag KQH32_RS11485 Protein ID WP_004155593.1
Coordinates 2568719..2568985 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQH32_RS11465 (KQH32_11490) 2565192..2565921 + 730 Protein_2218 SDR family oxidoreductase -
KQH32_RS11470 (KQH32_11495) 2566026..2566634 - 609 WP_004155587.1 HD domain-containing protein -
KQH32_RS11475 (KQH32_11500) 2566847..2567506 + 660 WP_004155588.1 hemolysin III family protein -
KQH32_RS11480 (KQH32_11505) 2567507..2568493 - 987 WP_004155590.1 tRNA-modifying protein YgfZ -
KQH32_RS11485 (KQH32_11510) 2568719..2568985 + 267 WP_004155593.1 FAD assembly factor SdhE Antitoxin
KQH32_RS11490 (KQH32_11515) 2568966..2569385 + 420 WP_004161808.1 protein YgfX Toxin
KQH32_RS11495 (KQH32_11520) 2569503..2570021 - 519 WP_004155596.1 flavodoxin FldB -
KQH32_RS11500 (KQH32_11525) 2570251..2571204 - 954 WP_004155597.1 DMT family transporter -
KQH32_RS11505 (KQH32_11530) 2571714..2573393 - 1680 WP_004155599.1 type III effector -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(8-79)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 16366.46 Da        Isoelectric Point: 11.9444

>T206028 WP_004161808.1 NZ_CP076596:2568966-2569385 [Erwinia amylovora]
VVLWQCELRPSRLAQRFSLLLHGAVMLALLLPTWPASSGLVRMLLLVLVLLECIRSRRRIGRRQGDIALLEGHELRWRQR
EWCIISRPWLTGQAILLLLQDTHGQRERLWLFADGMEKRNWRQLRLQLLNSKVQGNGWC

Download         Length: 420 bp

>T206028 NZ_CP102073:c5347-4484 [Enterococcus faecalis]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGATCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGAAATGTTGTTATCATTGATAATGATACGTTCAAACAACAGCACCCAAATTTTGACGAACTAGTG
AAACTCTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTAAG
CGATCAAGGCTATAATTTAGTGATTGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTACAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATAGCAGTACCGAAAATCAACTCATATTTAGGAACAATTGAACGGTATGAA
ACTATGTATGCAGATGATCCAATGACAGCTAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GCTTAGAAACGCCTTCCATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGGTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
ATTGGAAAGCTTGCAGCCACCTACACCACCAATACCAAAAATACCTAAGCTTCCAGGACTTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10498.07 Da        Isoelectric Point: 6.4605

>AT206028 WP_004155593.1 NZ_CP076596:2568719-2568985 [Erwinia amylovora]
MDITNKSRIHWACRRGMRELDISIMPFFQYEFDSLNDSDKRLFVRLLESDDPDLFNWLMNHGEPADVELKRMVALIQQRN
KQRGPVAM

Download         Length: 267 bp

>AT206028 NZ_CP102073:c5621-5349 [Enterococcus faecalis]
ATGGCAGTTACATATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A830ZTB8

Antitoxin

Source ID Structure
AlphaFold DB A0A831A3F0

References