Detailed information of TA system TA205999

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 2939749..2940376
• Replicon: chromosome
• Accession: NZ_CP076592
• Organism: Erwinia amylovora isolate CP20140001

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: D2T444
• Locus tag: KQH33_RS13215
• Protein ID: WP_004156337.1
• Coordinates: 2939749..2939967 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: KQH33_RS13220
• Protein ID: WP_013035913.1
• Coordinates: 2939987..2940376 (-)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQH33_RS13185 (KQH33_13215)	2935735..2936073	+	339	WP_004156319.1	P-II family nitrogen regulator	-
KQH33_RS13190 (KQH33_13220)	2936091..2937392	+	1302	WP_004172214.1	ammonium transporter AmtB	-
KQH33_RS13195 (KQH33_13225)	2937467..2938327	-	861	WP_004156324.1	acyl-CoA thioesterase II	-
KQH33_RS13200 (KQH33_13230)	2938552..2939109	+	558	WP_004156333.1	YbaY family lipoprotein	-
KQH33_RS13205 (KQH33_13235)	2939132..2939449	-	318	WP_004156334.1	MGMT family protein	-
KQH33_RS13215 (KQH33_13245)	2939749..2939967	-	219	WP_004156337.1	HHA domain-containing protein	Toxin
KQH33_RS13220 (KQH33_13250)	2939987..2940376	-	390	WP_013035913.1	Hha toxicity modulator TomB	Antitoxin
KQH33_RS13225 (KQH33_13255)	2940523..2940876	-	354	WP_004156342.1	hypothetical protein	-
KQH33_RS13230 (KQH33_13260)	2941648..2941794	-	147	WP_004156349.1	type B 50S ribosomal protein L36	-
KQH33_RS13235 (KQH33_13265)	2941782..2942063	-	282	WP_004156350.1	type B 50S ribosomal protein L31	-
KQH33_RS13240 (KQH33_13270)	2942211..2945357	-	3147	WP_004161886.1	multidrug efflux RND transporter permease subunit AcrB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8627.06 Da
• Isoelectric Point: 8.9007

>T205999 WP_004156337.1 NZ_CP076592:c2939967-2939749 [Erwinia amylovora]
MTDKLLTKTDYLMRLRRCRSIDTLERVIEKNKYELSDDELAVFYSAADHRLAELTMNKLYDKVPVAVWKFVR

Nucleotide

• Length: 219 bp

>T205999 NZ_CP102061:3355935-3356108 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTTTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 14965.70 Da
• Isoelectric Point: 4.7040

>AT205999 WP_013035913.1 NZ_CP076592:c2940376-2939987 [Erwinia amylovora]
MDEYSPKRHDIAQLKFLCENLYDESLATLGDSHHGWVNDPTSTSNLQLNDLIEHIAAFTMNYKIKHIEDSDLISQIDEYL
DDTFMLFSNYGVNNLDLQRWQKSAKRLFNIFAKECVMSQIQSSHSFSSP

Nucleotide

• Length: 390 bp

>AT205999 NZ_CP102061:3355611-3355907 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A831EPQ0