Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 65462..65984 Replicon plasmid pK186_2
Accession NZ_CP076520
Organism Klebsiella pneumoniae strain K186

Toxin (Protein)


Gene name relE Uniprot ID D0QMR1
Locus tag KNW01_RS27865 Protein ID WP_000220561.1
Coordinates 65703..65984 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag KNW01_RS27860 Protein ID WP_000121743.1
Coordinates 65462..65713 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KNW01_RS27840 (61585) 61585..61938 - 354 WP_024129958.1 DNA distortion polypeptide 3 -
KNW01_RS27845 (62075) 62075..62521 - 447 WP_001074384.1 hypothetical protein -
KNW01_RS27850 (62525) 62525..63367 - 843 WP_000435064.1 replication initiation protein -
KNW01_RS27855 (63388) 63388..64227 - 840 WP_024129959.1 replication initiation protein -
KNW01_RS27860 (65462) 65462..65713 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
KNW01_RS27865 (65703) 65703..65984 + 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KNW01_RS27870 (66223) 66223..66315 - 93 Protein_92 IS6 family transposase -
KNW01_RS27875 (66481) 66481..67680 - 1200 WP_000948429.1 IS91 family transposase -
KNW01_RS27880 (67690) 67690..67878 - 189 WP_000957857.1 hypothetical protein -
KNW01_RS27885 (67994) 67994..68788 - 795 Protein_95 TraD N-terminal domain-containing protein -
KNW01_RS27890 (68839) 68839..69576 - 738 WP_000199905.1 hypothetical protein -
KNW01_RS27895 (69779) 69779..70510 - 732 WP_000782451.1 conjugal transfer complement resistance protein TraT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid rmtB - 1..78128 78128
- flank IS/Tn - - 66481..67680 1199


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11023.86 Da        Isoelectric Point: 10.5938

>T205911 WP_000220561.1 NZ_CP076520:65703-65984 [Klebsiella pneumoniae]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Download         Length: 282 bp

>T205911 NZ_CP101925:197055-197162 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT205911 WP_000121743.1 NZ_CP076520:65462-65713 [Klebsiella pneumoniae]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT205911 NZ_CP101925:c197006-196943 [Escherichia coli]
GTCTAGGTTCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S5I302

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References