Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2610747..2611372 Replicon chromosome
Accession NZ_CP076285
Organism Escherichia coli strain 41

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag A7A12_RS12650 Protein ID WP_000911329.1
Coordinates 2610747..2611145 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag A7A12_RS12655 Protein ID WP_000450524.1
Coordinates 2611145..2611372 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A12_RS12630 (2606654) 2606654..2606854 + 201 WP_000383836.1 YpfN family protein -
A7A12_RS12635 (2606935) 2606935..2607633 - 699 WP_000679812.1 esterase -
A7A12_RS12640 (2607707) 2607707..2609722 - 2016 WP_000829332.1 tRNA cytosine(34) acetyltransferase TmcA -
A7A12_RS12645 (2609737) 2609737..2610600 - 864 WP_001267495.1 neutral zinc metallopeptidase -
A7A12_RS12650 (2610747) 2610747..2611145 - 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
A7A12_RS12655 (2611145) 2611145..2611372 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
A7A12_RS12660 (2611527) 2611527..2612240 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
A7A12_RS12665 (2612453) 2612453..2613487 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
A7A12_RS12670 (2613504) 2613504..2614382 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
A7A12_RS12675 (2614528) 2614528..2615100 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
A7A12_RS12680 (2615100) 2615100..2615570 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T205387 WP_000911329.1 NZ_CP076285:c2611145-2610747 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T205387 NZ_CP101615:769685-769981 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATACCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT205387 WP_000450524.1 NZ_CP076285:c2611372-2611145 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT205387 NZ_CP101615:769983-770378 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References