Detailed information of TA system TA205260

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 466540..467377
• Replicon: chromosome
• Accession: NZ_CP076276
• Organism: Escherichia coli strain SA31-1

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: A7A21_RS02150
• Protein ID: WP_000227784.1
• Coordinates: 466540..467082 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: A7A21_RS02155
• Protein ID: WP_001297137.1
• Coordinates: 467066..467377 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A21_RS02130 (462079)	462079..462990	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
A7A21_RS02135 (463158)	463158..463649	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
A7A21_RS02140 (463777)	463777..465141	-	1365	WP_001000955.1	MFS transporter	-
A7A21_RS02145 (465549)	465549..466484	+	936	WP_001297127.1	tetratricopeptide repeat protein	-
A7A21_RS02150 (466540)	466540..467082	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
A7A21_RS02155 (467066)	467066..467377	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
A7A21_RS02160 (467562)	467562..468452	-	891	WP_000971336.1	heme o synthase	-
A7A21_RS02165 (468464)	468464..468793	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
A7A21_RS02170 (468793)	468793..469407	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
A7A21_RS02175 (469397)	469397..471388	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
A7A21_RS02180 (471410)	471410..472357	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T205260 WP_000227784.1 NZ_CP076276:c467082-466540 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T205260 NZ_CP101533:878946-879353 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT205260 WP_001297137.1 NZ_CP076276:c467377-467066 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT205260 NZ_CP101533:878699-878965 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3