Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 466540..467377 Replicon chromosome
Accession NZ_CP076276
Organism Escherichia coli strain SA31-1

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag A7A21_RS02150 Protein ID WP_000227784.1
Coordinates 466540..467082 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag A7A21_RS02155 Protein ID WP_001297137.1
Coordinates 467066..467377 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A21_RS02130 (462079) 462079..462990 - 912 WP_000705874.1 2-dehydropantoate 2-reductase -
A7A21_RS02135 (463158) 463158..463649 + 492 WP_001138904.1 nucleotide binding protein YajQ -
A7A21_RS02140 (463777) 463777..465141 - 1365 WP_001000955.1 MFS transporter -
A7A21_RS02145 (465549) 465549..466484 + 936 WP_001297127.1 tetratricopeptide repeat protein -
A7A21_RS02150 (466540) 466540..467082 - 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
A7A21_RS02155 (467066) 467066..467377 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
A7A21_RS02160 (467562) 467562..468452 - 891 WP_000971336.1 heme o synthase -
A7A21_RS02165 (468464) 468464..468793 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
A7A21_RS02170 (468793) 468793..469407 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
A7A21_RS02175 (469397) 469397..471388 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
A7A21_RS02180 (471410) 471410..472357 - 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T205260 WP_000227784.1 NZ_CP076276:c467082-466540 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T205260 NZ_CP101533:878946-879353 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT205260 WP_001297137.1 NZ_CP076276:c467377-467066 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT205260 NZ_CP101533:878699-878965 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References