Detailed information of TA system TA205253

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 58491..59116
• Replicon: plasmid pP393F10IncF
• Accession: NZ_CP076275
• Organism: Escherichia coli strain P393-F10

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: A7A10_RS25480
• Protein ID: WP_000911315.1
• Coordinates: 58718..59116 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L4J1D2
• Locus tag: A7A10_RS25475
• Protein ID: WP_000450532.1
• Coordinates: 58491..58718 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A10_RS25475 (58491)	58491..58718	+	228	WP_000450532.1	toxin-antitoxin system antitoxin VapB	Antitoxin
A7A10_RS25480 (58718)	58718..59116	+	399	WP_000911315.1	type II toxin-antitoxin system VapC family toxin	Toxin
A7A10_RS25485 (59125)	59125..61323	-	2199	WP_257185970.1	type IV conjugative transfer system coupling protein TraD	-
A7A10_RS25490 (61575)	61575..62285	-	711	WP_042032882.1	conjugal transfer complement resistance protein TraT	-
A7A10_RS25495 (62331)	62331..62852	-	522	WP_000632670.1	conjugal transfer entry exclusion protein TraS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..153041	153041

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14871.17 Da
• Isoelectric Point: 8.5264

>T205253 WP_000911315.1 NZ_CP076275:58718-59116 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQARPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T205253 NZ_CP101527:c332984-332097 [Alkalimarinus sediminis]
ATGCTGAAGCAACAAATTCGCCAAATCGTCCAAGAAAACCCTGACTATGCAGCTGTGACCCCCGTCATCGAAAAAGAGAT
CTTGCATCACGACATTATGGATGTACTGATCAAACAAGGTGCTATGCAGCAATTAACCTTTATCGGTGGCACCTCATTAC
GTATGTGCTACAACAGCAGCAGACTATCGGAAGATCTTGATTTCAATGGTGGTCATAATTTTAAACCCTCGGATTTTGAG
GGGTTAGAGTCCGAAATACAAACCTATATTCAGAACAAGTATGAAACAGACGTTTGGGTAAACAAACCCTCCGATAAGCG
GCAAGGAGATACCGTTTCTTGGAAAATTAGTATCATAAAAGAGGCTAGTCGTCCTGATTTACCACGGCAAAAAATGCATA
TAGACGTCTGTGCTATTCCGTCATTCGATATCGAAAAGCGTCCGTTAATCAATCACTACAACATCGTTGTACCGACAGAA
GGGATTTTGGTTCCGGTACAATCTTTACAAGAAATGCTAGCCGATAAACTGATCGCGTTAGCGTATCGGGCAAGACGTAT
CAAACCTCGTGATATTTGGGATATTGTTTGGATCAAGCAGCGCGGTACTAATTTATCTAAGATACTGGTTGAAAAGAAGC
TCATTGCCAGACAGAAGCAAACTGAAGACTTTCGTCAGGCACTGTCTGTTCAGCTGGGTAAGCTAATGTCAGAAGATGAA
GTACGTGACGATTTCAACATGGAAATGAGCCGCTTTATCCCAAAGCAAACCAAAGAAAGAACCCTCGATAACCCTGAGTA
CTGGCCGTATGTTCAGGGCGAGGTAAATGCCATTGTATCTGAATTACTTAATGACAAATCGCCCAAAAATCCATTTGATA
TGGGTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8512.55 Da
• Isoelectric Point: 4.5358

>AT205253 WP_000450532.1 NZ_CP076275:58491-58718 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT205253 NZ_CP101527:c333537-333013 [Alkalimarinus sediminis]
ATGACTAAATCAGATAAGCTATTAAACGCATTAGCGAAAGCGGTAAACGCTGGAGGTGGCATTCACAGTGCAACTGAGCT
GGCTTTTATGCTGGGCTTACCTTATGACCCTGCCTTTAGAAAGTTCCTAGCAGATTGTGTTAATAAAGGCTTACTCAGAC
GCGTGATGAAGGGCCTATATGAAAGCGCAATTACGCCACCTGAGCCTGAGACCGCCATTTATAAAATCATCAAAAAACTG
CGAAGTGGCGTATTGAACTATATCAGCTTAGAAAGTCAGCTCAGTCATACAGGCGATATTTCACAAGTCGTGATGGGTAG
AGTCACGGTGGTGACAAAAGGTCGAAGTGGTTGCTTTGATACGCCCTATGGCGTGATCGAATTGACCCATACTAAAAAGC
CAGTCGAGAAAATTGCACCTAATTTATACTATGATCCCGACATCAAAATGTATCGTGCGCACAAAGCCCAAGCAATTGCT
GATCTTAAGCACTGTCAGCGCAATTTACATATGTTGGAGAGCTAA

Structures

Antitoxin

• AlphaFold DB: A0A829CHF5