Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 502775..503393 Replicon chromosome
Accession NZ_CP076274
Organism Escherichia coli strain P393-F10

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag A7A10_RS02305 Protein ID WP_001291435.1
Coordinates 502775..502993 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag A7A10_RS02310 Protein ID WP_000344800.1
Coordinates 503019..503393 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A10_RS02270 (498064) 498064..498636 + 573 WP_000779831.1 YbaY family lipoprotein -
A7A10_RS02275 (498667) 498667..498978 - 312 WP_000409911.1 MGMT family protein -
A7A10_RS02285 (499357) 499357..499710 + 354 WP_000878140.1 DUF1428 domain-containing protein -
A7A10_RS02290 (499752) 499752..501302 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
A7A10_RS02295 (501466) 501466..501936 - 471 WP_000136192.1 YlaC family protein -
A7A10_RS02300 (502052) 502052..502603 - 552 WP_000102568.1 maltose O-acetyltransferase -
A7A10_RS02305 (502775) 502775..502993 - 219 WP_001291435.1 HHA domain-containing protein Toxin
A7A10_RS02310 (503019) 503019..503393 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
A7A10_RS02315 (503939) 503939..507088 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
A7A10_RS02320 (507111) 507111..508304 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T205217 WP_001291435.1 NZ_CP076274:c502993-502775 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T205217 NZ_CP101513:732218-732514 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT205217 WP_000344800.1 NZ_CP076274:c503393-503019 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT205217 NZ_CP101513:732516-732911 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References