Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3342642..3343296 Replicon chromosome
Accession NZ_CP076272
Organism Escherichia coli strain E21845/O/A

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag A7A11_RS16510 Protein ID WP_000244777.1
Coordinates 3342642..3343049 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag A7A11_RS16515 Protein ID WP_000354046.1
Coordinates 3343030..3343296 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A11_RS16490 (3338599) 3338599..3340332 - 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -
A7A11_RS16495 (3340338) 3340338..3341048 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
A7A11_RS16500 (3341073) 3341073..3341969 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
A7A11_RS16505 (3342081) 3342081..3342602 + 522 WP_001055874.1 flavodoxin FldB -
A7A11_RS16510 (3342642) 3342642..3343049 - 408 WP_000244777.1 protein YgfX Toxin
A7A11_RS16515 (3343030) 3343030..3343296 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
A7A11_RS16520 (3343539) 3343539..3344519 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
A7A11_RS16525 (3344596) 3344596..3345255 - 660 WP_000250274.1 hemolysin III family protein -
A7A11_RS16530 (3345419) 3345419..3345730 - 312 WP_115427368.1 N(4)-acetylcytidine aminohydrolase -
A7A11_RS16535 (3345775) 3345775..3347208 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
A7A11_RS16540 (3347265) 3347265..3348008 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T205206 WP_000244777.1 NZ_CP076272:c3343049-3342642 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T205206 NZ_CP101497:c41973-41677 [Microcella sp. MMS21-STM10]
ATGACGACTGAGAGGTTCAGCCAATTCGATGCTGCCGACTATCTGTCCGACATGGAGACCGCGGCGGCCTATCTCGAAGC
GACGATCGCCGAGAGCGGCGACGACCCTGCCACCATTTCGGCTGCGCTCGGGGCCATCGCCCGCTCGGGCAACCTCAGCG
AGCTGGCTCGGCGCACCGGGATGAGCCGCGAGGGGCTTTACAAGGCGCTCTCCGCCGACGGGAATCCGAGCTTCGCGACC
ATTGTCAAGGTGTCGAGGGCGCTCGGGCTCCGGTTGGAGTTTCACGCCATCGCCTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT205206 WP_000354046.1 NZ_CP076272:c3343296-3343030 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT205206 NZ_CP101497:c42274-41963 [Microcella sp. MMS21-STM10]
ATGTACGAGCTGCGCAGAAGTGCTGAGTTCGACCGGTGGTTGGCCAGGCTGAGCGACCGTGGTGCTGTTGCGCGGGTCTT
GGTACGGCTTGATCGCCTTGCACGCGGCAACCCCGGCGACGTTCGACCCGTTGGCTTCGGTGTATCGGAGCTACGGATCG
ACCATGGACCCGGTCTTCGCGTGTACTACCTGCAACACGGCGGCGCCATAGTGCTGCTGCTGTGCGGGGGTGACAAGTCG
ACTCAGTCGCGAGACATCAGGCGGGCACATCGTGTGGCCGAGCAATGGAGGGGAGAGAAGCATGACGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References