Detailed information of TA system TA205173

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3328386..3329221
• Replicon: chromosome
• Accession: NZ_CP076270
• Organism: Escherichia coli strain Ceylon31

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: A7A14_RS16190
• Protein ID: WP_157704300.1
• Coordinates: 3328844..3329221 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3Z2YIX3
• Locus tag: A7A14_RS16185
• Protein ID: WP_032178229.1
• Coordinates: 3328386..3328754 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A14_RS16160 (3325497)	3325497..3326315	+	819	WP_157704298.1	DUF932 domain-containing protein	-
A7A14_RS16165 (3326407)	3326407..3326892	+	486	WP_000213706.1	antirestriction protein	-
A7A14_RS16170 (3326907)	3326907..3327383	+	477	WP_001186784.1	RadC family protein	-
A7A14_RS16175 (3327452)	3327452..3327673	+	222	WP_000692353.1	DUF987 domain-containing protein	-
A7A14_RS16180 (3327692)	3327692..3328336	+	645	WP_157704299.1	antitoxin of toxin-antitoxin stability system	-
A7A14_RS16185 (3328386)	3328386..3328754	+	369	WP_032178229.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
A7A14_RS16190 (3328844)	3328844..3329221	+	378	WP_157704300.1	TA system toxin CbtA family protein	Toxin
A7A14_RS16195 (3329218)	3329218..3329706	+	489	WP_000779171.1	DUF5983 family protein	-
A7A14_RS16200 (3329718)	3329718..3329915	+	198	WP_001374283.1	DUF957 domain-containing protein	-
A7A14_RS16205 (3330000)	3330000..3330842	+	843	WP_032176805.1	DUF4942 domain-containing protein	-
A7A14_RS16210 (3331123)	3331123..3331659	-	537	WP_000942795.1	GspM family type II secretion system protein YghD	-
A7A14_RS16215 (3331661)	3331661..3332839	-	1179	WP_033547699.1	type II secretion system protein GspL	-
A7A14_RS16220 (3332836)	3332836..3333813	-	978	WP_000633238.1	type II secretion system minor pseudopilin GspK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14058.09 Da
• Isoelectric Point: 9.1376

>T205173 WP_157704300.1 NZ_CP076270:3328844-3329221 [Escherichia coli]
MKTLPVLPGQAAGLRSSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDKPGF
NACTHSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHSEAKR

Nucleotide

• Length: 378 bp

>T205173 NZ_CP101390:c4290105-4289821 [Salmonella enterica]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13823.58 Da
• Isoelectric Point: 6.3177

>AT205173 WP_032178229.1 NZ_CP076270:3328386-3328754 [Escherichia coli]
VSDTLHETNYPDDNNDRSWWGLPCTVTPCFRARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT205173 NZ_CP101390:c4290337-4290095 [Salmonella enterica]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A3Z2YIX3