Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 277019..277560 Replicon chromosome
Accession NZ_CP076270
Organism Escherichia coli strain Ceylon31

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag A7A14_RS01235 Protein ID WP_000615976.1
Coordinates 277019..277297 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag A7A14_RS01240 Protein ID WP_000729704.1
Coordinates 277300..277560 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A14_RS01220 (274601) 274601..275179 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
A7A14_RS01225 (275385) 275385..276152 + 768 WP_000333380.1 class II glutamine amidotransferase -
A7A14_RS01230 (276123) 276123..276863 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
A7A14_RS01235 (277019) 277019..277297 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
A7A14_RS01240 (277300) 277300..277560 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
A7A14_RS01245 (277746) 277746..278519 + 774 WP_000543899.1 C40 family peptidase -
A7A14_RS01250 (278576) 278576..278932 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
A7A14_RS01255 (278925) 278925..279203 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
A7A14_RS01260 (279308) 279308..281020 - 1713 Protein_244 flagellar biosynthesis protein FlhA -
A7A14_RS01265 (280992) 280992..281777 + 786 WP_000207574.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T205153 WP_000615976.1 NZ_CP076270:c277297-277019 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T205153 NZ_CP101388:2471815-2472099 [Salmonella enterica]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGGCTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGACACAGAAGCTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT205153 WP_000729704.1 NZ_CP076270:c277560-277300 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT205153 NZ_CP101388:2471577-2471825 [Salmonella enterica]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGCAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References