Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 3285864..3286659 | Replicon | chromosome |
| Accession | NZ_CP076243 | ||
| Organism | Escherichia coli O157:H7 strain Balcarce_14.2 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | B7UP43 |
| Locus tag | JKN69_RS17055 | Protein ID | WP_000854914.1 |
| Coordinates | 3285864..3286238 (-) | Length | 125 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | A0A0B0VS41 |
| Locus tag | JKN69_RS17060 | Protein ID | WP_001280954.1 |
| Coordinates | 3286285..3286659 (-) | Length | 125 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| JKN69_RS17015 (3280868) | 3280868..3281359 | - | 492 | WP_001301587.1 | DUF1097 domain-containing protein | - |
| JKN69_RS17020 (3281461) | 3281461..3282015 | - | 555 | WP_001001917.1 | molecular chaperone YcdY | - |
| JKN69_RS17025 (3282039) | 3282039..3282776 | - | 738 | WP_000283664.1 | zinc-binding phosphatase | - |
| JKN69_RS17030 (3282831) | 3282831..3283769 | - | 939 | WP_000351284.1 | glyoxylate/hydroxypyruvate reductase GhrA | - |
| JKN69_RS17040 (3284240) | 3284240..3285082 | - | 843 | WP_001280481.1 | DUF4942 domain-containing protein | - |
| JKN69_RS17045 (3285167) | 3285167..3285364 | - | 198 | WP_000839282.1 | DUF957 domain-containing protein | - |
| JKN69_RS17050 (3285376) | 3285376..3285867 | - | 492 | WP_000976853.1 | DUF5983 family protein | - |
| JKN69_RS17055 (3285864) | 3285864..3286238 | - | 375 | WP_000854914.1 | TA system toxin CbtA family protein | Toxin |
| JKN69_RS17060 (3286285) | 3286285..3286659 | - | 375 | WP_001280954.1 | type IV toxin-antitoxin system YeeU family antitoxin | Antitoxin |
| JKN69_RS17065 (3286822) | 3286822..3287043 | - | 222 | WP_001220314.1 | DUF987 domain-containing protein | - |
| JKN69_RS17070 (3287106) | 3287106..3287582 | - | 477 | WP_001186738.1 | RadC family protein | - |
| JKN69_RS17075 (3287598) | 3287598..3288083 | - | 486 | WP_000214398.1 | antirestriction protein | - |
| JKN69_RS17080 (3288174) | 3288174..3288992 | - | 819 | WP_001234682.1 | DUF932 domain-containing protein | - |
| JKN69_RS17085 (3289332) | 3289332..3290402 | - | 1071 | WP_000102669.1 | patatin-like phospholipase family protein | - |
| JKN69_RS17090 (3290399) | 3290399..3291304 | - | 906 | WP_000203541.1 | diguanylate cyclase regulator RdcB family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 125 a.a. Molecular weight: 14117.17 Da Isoelectric Point: 7.7761
>T204946 WP_000854914.1 NZ_CP076243:c3286238-3285864 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
Download Length: 375 bp
>T204946 NZ_CP101302:c4467331-4466924 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA
Antitoxin
Download Length: 125 a.a. Molecular weight: 13718.51 Da Isoelectric Point: 6.6249
>AT204946 WP_001280954.1 NZ_CP076243:c3286659-3286285 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS
Download Length: 375 bp
>AT204946 NZ_CP101302:c4467578-4467312 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7U9LXR5 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0B0VS41 |