Detailed information of TA system TA204922

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4635708..4636258
• Replicon: chromosome
• Accession: NZ_CP076241
• Organism: Escherichia coli O157:H7 strain Vac_07.1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: JKN65_RS23430
• Protein ID: WP_001160975.1
• Coordinates: 4635708..4636022 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: JKN65_RS23435
• Protein ID: WP_000125566.1
• Coordinates: 4636025..4636258 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKN65_RS23405 (4631383)	4631383..4632669	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
JKN65_RS23410 (4632669)	4632669..4633658	+	990	WP_000241259.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
JKN65_RS23415 (4633655)	4633655..4634476	+	822	WP_001065380.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
JKN65_RS23420 (4634479)	4634479..4634856	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
JKN65_RS23425 (4634863)	4634863..4635711	+	849	WP_000257187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
JKN65_RS23430 (4635708)	4635708..4636022	-	315	WP_001160975.1	CcdB family protein	Toxin
JKN65_RS23435 (4636025)	4636025..4636258	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
JKN65_RS23440 (4636344)	4636344..4636823	-	480	WP_000624372.1	type 3 dihydrofolate reductase	-
JKN65_RS23445 (4637015)	4637015..4638877	-	1863	WP_000377184.1	glutathione-regulated potassium-efflux system protein KefC	-
JKN65_RS23450 (4638870)	4638870..4639400	-	531	WP_000600700.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
JKN65_RS23455 (4639508)	4639508..4640839	-	1332	WP_001183177.1	MFS transporter	-
JKN65_RS23460 (4640897)	4640897..4641184	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T204922 WP_001160975.1 NZ_CP076241:c4636022-4635708 [Escherichia coli O157:H7]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T204922 NZ_CP101292:c4643592-4643281 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCATCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT204922 WP_000125566.1 NZ_CP076241:c4636258-4636025 [Escherichia coli O157:H7]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT204922 NZ_CP101292:c4643284-4642865 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9