Detailed information of TA system TA204887

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3554453..3554678
• Replicon: chromosome
• Accession: NZ_CP076237
• Organism: Escherichia coli O157:H7 strain 146N4

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: JKN67_RS18400
• Protein ID: WP_000813263.1
• Coordinates: 3554453..3554608 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3554620..3554678 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKN67_RS18365	3549907..3550620	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
JKN67_RS18370	3550758..3550954	-	197	Protein_3591	TrmB family transcriptional regulator	-
JKN67_RS18375	3551241..3552059	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
JKN67_RS18380	3552211..3552582	-	372	WP_000090264.1	antiterminator Q family protein	-
JKN67_RS18385	3552572..3552943	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
JKN67_RS18390	3552956..3554005	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
JKN67_RS18395	3554007..3554285	-	279	WP_001341388.1	hypothetical protein	-
JKN67_RS18400	3554453..3554608	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3554620..3554678	+	59	-	-	Antitoxin
JKN67_RS18405	3554868..3554951	-	84	Protein_3598	ORF6N domain-containing protein	-
JKN67_RS18410	3555213..3555986	+	774	WP_000160654.1	alpha/beta hydrolase	-
JKN67_RS18415	3556338..3556751	-	414	WP_001151233.1	DUF977 family protein	-
JKN67_RS18420	3556767..3557537	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
JKN67_RS18425	3557559..3558305	-	747	WP_000788751.1	ATP-binding protein	-
JKN67_RS18430	3558312..3559403	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T204887 WP_000813263.1 NZ_CP076237:c3554608-3554453 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T204887 NZ_CP101274:c4373150-4372920 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT204887 NZ_CP076237:3554620-3554678 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin