Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3336700..3337495 Replicon chromosome
Accession NZ_CP076237
Organism Escherichia coli O157:H7 strain 146N4

Toxin (Protein)


Gene name yeeV Uniprot ID B7UP43
Locus tag JKN67_RS17260 Protein ID WP_000854914.1
Coordinates 3336700..3337074 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0B0VS41
Locus tag JKN67_RS17265 Protein ID WP_001280954.1
Coordinates 3337121..3337495 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKN67_RS17220 (3331704) 3331704..3332195 - 492 WP_001301587.1 DUF1097 domain-containing protein -
JKN67_RS17225 (3332297) 3332297..3332851 - 555 WP_001001917.1 molecular chaperone YcdY -
JKN67_RS17230 (3332875) 3332875..3333612 - 738 WP_000283664.1 zinc-binding phosphatase -
JKN67_RS17235 (3333667) 3333667..3334605 - 939 WP_000351284.1 glyoxylate/hydroxypyruvate reductase GhrA -
JKN67_RS17245 (3335076) 3335076..3335918 - 843 WP_001280481.1 DUF4942 domain-containing protein -
JKN67_RS17250 (3336003) 3336003..3336200 - 198 WP_000839282.1 DUF957 domain-containing protein -
JKN67_RS17255 (3336212) 3336212..3336703 - 492 WP_000976853.1 DUF5983 family protein -
JKN67_RS17260 (3336700) 3336700..3337074 - 375 WP_000854914.1 TA system toxin CbtA family protein Toxin
JKN67_RS17265 (3337121) 3337121..3337495 - 375 WP_001280954.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
JKN67_RS17270 (3337658) 3337658..3337879 - 222 WP_001220314.1 DUF987 domain-containing protein -
JKN67_RS17275 (3337942) 3337942..3338418 - 477 WP_001186738.1 RadC family protein -
JKN67_RS17280 (3338434) 3338434..3338919 - 486 WP_000214398.1 antirestriction protein -
JKN67_RS17285 (3339010) 3339010..3339828 - 819 WP_001234682.1 DUF932 domain-containing protein -
JKN67_RS17290 (3340168) 3340168..3341238 - 1071 WP_000102669.1 patatin-like phospholipase family protein -
JKN67_RS17295 (3341235) 3341235..3342140 - 906 WP_000203541.1 diguanylate cyclase regulator RdcB family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - csgB / csgD / csgE / csgF / csgG 3328086..3338919 10833


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14117.17 Da        Isoelectric Point: 7.7761

>T204886 WP_000854914.1 NZ_CP076237:c3337074-3336700 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T204886 NZ_CP101274:c4351584-4351057 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13718.51 Da        Isoelectric Point: 6.6249

>AT204886 WP_001280954.1 NZ_CP076237:c3337495-3337121 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Download         Length: 375 bp

>AT204886 NZ_CP101274:c4351857-4351591 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LXR5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0VS41

References