Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 3089345..3089983 Replicon chromosome
Accession NZ_CP076237
Organism Escherichia coli O157:H7 strain 146N4

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag JKN67_RS15825 Protein ID WP_000813794.1
Coordinates 3089807..3089983 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag JKN67_RS15820 Protein ID WP_001270286.1
Coordinates 3089345..3089761 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKN67_RS15800 (3084497) 3084497..3085438 - 942 WP_001251315.1 ABC transporter permease -
JKN67_RS15805 (3085439) 3085439..3086452 - 1014 WP_000220402.1 ABC transporter ATP-binding protein -
JKN67_RS15810 (3086470) 3086470..3087615 - 1146 WP_000047432.1 ABC transporter substrate-binding protein -
JKN67_RS15815 (3087860) 3087860..3089266 - 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
JKN67_RS15820 (3089345) 3089345..3089761 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
JKN67_RS15825 (3089807) 3089807..3089983 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
JKN67_RS15830 (3090205) 3090205..3090435 + 231 WP_000494244.1 YncJ family protein -
JKN67_RS15835 (3090527) 3090527..3092488 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
JKN67_RS15840 (3092561) 3092561..3093097 - 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
JKN67_RS15845 (3093150) 3093150..3094361 + 1212 WP_071525382.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T204883 WP_000813794.1 NZ_CP076237:c3089983-3089807 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T204883 NZ_CP101274:c3640010-3639675 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT204883 WP_001270286.1 NZ_CP076237:c3089761-3089345 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT204883 NZ_CP101274:c3640258-3640010 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References