Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3264261..3265056 Replicon chromosome
Accession NZ_CP076235
Organism Escherichia coli O157:H7 strain 9.1_Anguil

Toxin (Protein)


Gene name yeeV Uniprot ID B7UP43
Locus tag JKN68_RS16870 Protein ID WP_000854914.1
Coordinates 3264261..3264635 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0B0VS41
Locus tag JKN68_RS16875 Protein ID WP_001280954.1
Coordinates 3264682..3265056 (-) Length 125 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKN68_RS16830 (3259265) 3259265..3259756 - 492 WP_001301587.1 DUF1097 domain-containing protein -
JKN68_RS16835 (3259858) 3259858..3260412 - 555 WP_001001917.1 molecular chaperone YcdY -
JKN68_RS16840 (3260436) 3260436..3261173 - 738 WP_000283664.1 zinc-binding phosphatase -
JKN68_RS16845 (3261228) 3261228..3262166 - 939 WP_000351284.1 glyoxylate/hydroxypyruvate reductase GhrA -
JKN68_RS16855 (3262637) 3262637..3263479 - 843 WP_001280481.1 DUF4942 domain-containing protein -
JKN68_RS16860 (3263564) 3263564..3263761 - 198 WP_000839282.1 DUF957 domain-containing protein -
JKN68_RS16865 (3263773) 3263773..3264264 - 492 WP_000976853.1 DUF5983 family protein -
JKN68_RS16870 (3264261) 3264261..3264635 - 375 WP_000854914.1 TA system toxin CbtA family protein Toxin
JKN68_RS16875 (3264682) 3264682..3265056 - 375 WP_001280954.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
JKN68_RS16880 (3265219) 3265219..3265440 - 222 WP_001220314.1 DUF987 domain-containing protein -
JKN68_RS16885 (3265503) 3265503..3265979 - 477 WP_001186738.1 RadC family protein -
JKN68_RS16890 (3265995) 3265995..3266480 - 486 WP_000214398.1 antirestriction protein -
JKN68_RS16895 (3266571) 3266571..3267389 - 819 WP_001234682.1 DUF932 domain-containing protein -
JKN68_RS16900 (3267729) 3267729..3268799 - 1071 WP_000102669.1 patatin-like phospholipase family protein -
JKN68_RS16905 (3268796) 3268796..3269701 - 906 WP_000203541.1 diguanylate cyclase regulator RdcB family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - csgB / csgD / csgE / csgF / csgG 3255647..3266480 10833


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-112)

Antitoxin

(18-117)


Sequences


Toxin        


Download         Length: 125 a.a.        Molecular weight: 14117.17 Da        Isoelectric Point: 7.7761

>T204849 WP_000854914.1 NZ_CP076235:c3264635-3264261 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T204849 NZ_CP101269:1849280-1849534 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13718.51 Da        Isoelectric Point: 6.6249

>AT204849 WP_001280954.1 NZ_CP076235:c3265056-3264682 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Download         Length: 375 bp

>AT204849 NZ_CP101269:1849032-1849283 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7U9LXR5


Antitoxin

Source ID Structure
AlphaFold DB A0A0B0VS41

References