Detailed information of TA system TA204825

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 5435709..5436508
• Replicon: chromosome
• Accession: NZ_CP076232
• Organism: Escherichia coli O157:H7 strain 7.1_Anguil

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: XE90_RS27430
• Protein ID: WP_000347273.1
• Coordinates: 5436044..5436508 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: XE90_RS27425
• Protein ID: WP_001302819.1
• Coordinates: 5435709..5436044 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XE90_RS27410 (5431461)	5431461..5432231	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
XE90_RS27415 (5432247)	5432247..5433581	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
XE90_RS27420 (5433956)	5433956..5435560	+	1605	WP_046425983.1	galactarate dehydratase	-
XE90_RS27425 (5435709)	5435709..5436044	+	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
XE90_RS27430 (5436044)	5436044..5436508	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
XE90_RS27435 (5436563)	5436563..5437372	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
XE90_RS27440 (5437621)	5437621..5438901	+	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
XE90_RS27445 (5438924)	5438924..5439397	+	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
XE90_RS27450 (5439408)	5439408..5440187	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
XE90_RS27455 (5440177)	5440177..5441055	+	879	Protein_5355	PTS N-acetylgalactosamine transporter subunit IID	-
XE90_RS27460 (5441073)	5441073..5441507	+	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T204825 WP_000347273.1 NZ_CP076232:5436044-5436508 [Escherichia coli O157:H7]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T204825 NZ_CP101263:c795374-794997 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCATACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACCGAACAGTAAATAACATTACTCTGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT204825 WP_001302819.1 NZ_CP076232:5435709-5436044 [Escherichia coli O157:H7]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT204825 NZ_CP101263:c795795-795421 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGGCCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: V0SSH7