Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 4186556..4187194 Replicon chromosome
Accession NZ_CP076232
Organism Escherichia coli O157:H7 strain 7.1_Anguil

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag XE90_RS21205 Protein ID WP_000813794.1
Coordinates 4187018..4187194 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag XE90_RS21200 Protein ID WP_001270286.1
Coordinates 4186556..4186972 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
XE90_RS21180 (4181708) 4181708..4182649 - 942 WP_001251315.1 ABC transporter permease -
XE90_RS21185 (4182650) 4182650..4183663 - 1014 WP_000220402.1 ABC transporter ATP-binding protein -
XE90_RS21190 (4183681) 4183681..4184826 - 1146 WP_000047432.1 ABC transporter substrate-binding protein -
XE90_RS21195 (4185071) 4185071..4186477 - 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
XE90_RS21200 (4186556) 4186556..4186972 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
XE90_RS21205 (4187018) 4187018..4187194 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
XE90_RS21210 (4187416) 4187416..4187646 + 231 WP_000494244.1 YncJ family protein -
XE90_RS21215 (4187738) 4187738..4189699 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
XE90_RS21220 (4189772) 4189772..4190308 - 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
XE90_RS21225 (4190361) 4190361..4191572 + 1212 WP_071525382.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T204820 WP_000813794.1 NZ_CP076232:c4187194-4187018 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T204820 NZ_CP101260:c52264-51884 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT204820 WP_001270286.1 NZ_CP076232:c4186972-4186556 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT204820 NZ_CP101260:c52485-52264 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References