Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 56498..57057 | Replicon | plasmid pO157 |
| Accession | NZ_CP076231 | ||
| Organism | Escherichia coli O157:H7 strain Rafaela_II | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | O82928 |
| Locus tag | XF37_RS28030 | Protein ID | WP_000421248.1 |
| Coordinates | 56782..57057 (+) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | C1J8K5 |
| Locus tag | XF37_RS28025 | Protein ID | WP_001178089.1 |
| Coordinates | 56498..56782 (+) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| XF37_RS27985 (51691) | 51691..52251 | + | 561 | WP_000840472.1 | fertility inhibition protein FinO | - |
| XF37_RS27990 (52384) | 52384..52596 | + | 213 | WP_001302189.1 | ANR family transcriptional regulator | - |
| XF37_RS27995 (52841) | 52841..53302 | + | 462 | WP_001233856.1 | thermonuclease family protein | - |
| XF37_RS28000 (53348) | 53348..53557 | + | 210 | WP_001302200.1 | hemolysin expression modulator Hha | - |
| XF37_RS28005 (53595) | 53595..53933 | + | 339 | WP_000766796.1 | hypothetical protein | - |
| XF37_RS28010 (54173) | 54173..54427 | + | 255 | WP_000083831.1 | replication regulatory protein RepA | - |
| XF37_RS28015 (54663) | 54663..54737 | + | 75 | WP_001370046.1 | RepA leader peptide Tap | - |
| XF37_RS28020 (54730) | 54730..55587 | + | 858 | WP_000130945.1 | incFII family plasmid replication initiator RepA | - |
| XF37_RS28025 (56498) | 56498..56782 | + | 285 | WP_001178089.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| XF37_RS28030 (56782) | 56782..57057 | + | 276 | WP_000421248.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| XF37_RS28035 (57152) | 57152..57358 | + | 207 | WP_001105064.1 | hypothetical protein | - |
| XF37_RS28040 (57458) | 57458..57673 | - | 216 | Protein_65 | transposase | - |
| XF37_RS28045 (57717) | 57717..58283 | - | 567 | Protein_66 | IS3-like element IS600 family transposase | - |
| XF37_RS28050 (58283) | 58283..58699 | - | 417 | Protein_67 | IS91 family transposase | - |
| XF37_RS28055 (58699) | 58699..58884 | - | 186 | WP_001302179.1 | hypothetical protein | - |
| XF37_RS28060 (59061) | 59061..61271 | + | 2211 | WP_000592771.1 | catalase/peroxidase KatP | - |
| XF37_RS28065 (61315) | 61315..61704 | + | 390 | WP_001172748.1 | cytochrome b562 | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | hlyC / hlyA / hlyB / hlyB / hlyD / toxB / espP / stcE / exeE / exeG | 1..94756 | 94756 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10735.42 Da Isoelectric Point: 9.9324
>T204795 WP_000421248.1 NZ_CP076231:56782-57057 [Escherichia coli O157:H7]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T204795 NZ_CP101251:3415710-3415928 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA
Antitoxin
Download Length: 95 a.a. Molecular weight: 10474.89 Da Isoelectric Point: 5.6558
>AT204795 WP_001178089.1 NZ_CP076231:56498-56782 [Escherichia coli O157:H7]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
Download Length: 285 bp
>AT204795 NZ_CP101251:3415310-3415684 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2Z6FAZ0 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A731IHA8 |