Detailed information of TA system TA204789

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4738214..4738764
• Replicon: chromosome
• Accession: NZ_CP076230
• Organism: Escherichia coli O157:H7 strain Rafaela_II

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: XF37_RS24025
• Protein ID: WP_001160975.1
• Coordinates: 4738214..4738528 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: XF37_RS24030
• Protein ID: WP_000125566.1
• Coordinates: 4738531..4738764 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XF37_RS24000 (4733889)	4733889..4735175	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
XF37_RS24005 (4735175)	4735175..4736164	+	990	WP_000241259.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
XF37_RS24010 (4736161)	4736161..4736982	+	822	WP_001065380.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
XF37_RS24015 (4736985)	4736985..4737362	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
XF37_RS24020 (4737369)	4737369..4738217	+	849	WP_000257187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
XF37_RS24025 (4738214)	4738214..4738528	-	315	WP_001160975.1	CcdB family protein	Toxin
XF37_RS24030 (4738531)	4738531..4738764	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
XF37_RS24035 (4738850)	4738850..4739329	-	480	WP_000624372.1	type 3 dihydrofolate reductase	-
XF37_RS24040 (4739521)	4739521..4741383	-	1863	WP_000377184.1	glutathione-regulated potassium-efflux system protein KefC	-
XF37_RS24045 (4741376)	4741376..4741906	-	531	WP_000600700.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
XF37_RS24050 (4742014)	4742014..4743345	-	1332	WP_001183177.1	MFS transporter	-
XF37_RS24055 (4743403)	4743403..4743690	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T204789 WP_001160975.1 NZ_CP076230:c4738528-4738214 [Escherichia coli O157:H7]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T204789 NZ_CP101251:1086228-1086539 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT204789 WP_000125566.1 NZ_CP076230:c4738764-4738531 [Escherichia coli O157:H7]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT204789 NZ_CP101251:1086536-1086955 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9