Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2796971..2797609 Replicon chromosome
Accession NZ_CP076230
Organism Escherichia coli O157:H7 strain Rafaela_II

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag XF37_RS14270 Protein ID WP_000813794.1
Coordinates 2797433..2797609 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag XF37_RS14265 Protein ID WP_001270286.1
Coordinates 2796971..2797387 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
XF37_RS14245 (2792123) 2792123..2793064 - 942 WP_001251315.1 ABC transporter permease -
XF37_RS14250 (2793065) 2793065..2794078 - 1014 WP_000220402.1 ABC transporter ATP-binding protein -
XF37_RS14255 (2794096) 2794096..2795241 - 1146 WP_000047432.1 ABC transporter substrate-binding protein -
XF37_RS14260 (2795486) 2795486..2796892 - 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
XF37_RS14265 (2796971) 2796971..2797387 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
XF37_RS14270 (2797433) 2797433..2797609 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
XF37_RS14275 (2797831) 2797831..2798061 + 231 WP_000494244.1 YncJ family protein -
XF37_RS14280 (2798153) 2798153..2800114 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
XF37_RS14285 (2800187) 2800187..2800723 - 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
XF37_RS14290 (2800815) 2800815..2801976 + 1162 Protein_2794 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T204782 WP_000813794.1 NZ_CP076230:c2797609-2797433 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T204782 NZ_CP101248:c52264-51884 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT204782 WP_001270286.1 NZ_CP076230:c2797387-2796971 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT204782 NZ_CP101248:c52485-52264 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References