Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2621973..2622444 Replicon chromosome
Accession NZ_CP076230
Organism Escherichia coli O157:H7 strain Rafaela_II

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag XF37_RS13395 Protein ID WP_001303511.1
Coordinates 2621973..2622251 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag XF37_RS13400 Protein ID WP_001302048.1
Coordinates 2622253..2622444 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
XF37_RS13365 (2617199) 2617199..2617450 - 252 WP_000005552.1 excisionase family protein -
XF37_RS13370 (2617523) 2617523..2619994 - 2472 WP_000048458.1 exonuclease -
XF37_RS13375 (2620087) 2620087..2620278 - 192 WP_001090200.1 DUF1482 family protein -
XF37_RS13380 (2620275) 2620275..2620463 - 189 WP_000449192.1 cell division inhibition protein DicB -
XF37_RS13385 (2621032) 2621032..2621250 - 219 WP_001171930.1 protein YdfC -
XF37_RS13390 (2621322) 2621322..2621621 - 300 WP_001240334.1 hypothetical protein -
XF37_RS13395 (2621973) 2621973..2622251 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
XF37_RS13400 (2622253) 2622253..2622444 - 192 WP_001302048.1 hypothetical protein Antitoxin
XF37_RS13405 (2622465) 2622465..2622836 - 372 WP_001169686.1 hypothetical protein -
XF37_RS13410 (2622934) 2622934..2623005 + 72 Protein_2622 hypothetical protein -
XF37_RS13415 (2623058) 2623058..2624271 + 1214 WP_162829202.1 IS3-like element IS1203 family transposase -
XF37_RS13420 (2624316) 2624316..2624549 + 234 Protein_2624 transcriptional regulator -
XF37_RS13425 (2624546) 2624546..2624971 + 426 WP_000693943.1 toxin YdaT family protein -
XF37_RS13430 (2624994) 2624994..2625956 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
XF37_RS13435 (2625963) 2625963..2626703 + 741 WP_000788939.1 ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2614813..2665742 50929


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T204781 WP_001303511.1 NZ_CP076230:c2622251-2621973 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T204781 NZ_CP101247:39081-39233 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT204781 WP_001302048.1 NZ_CP076230:c2622444-2622253 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT204781 NZ_CP101247:c39029-38969 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References