Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 417755..418555 Replicon chromosome
Accession NZ_CP076230
Organism Escherichia coli O157:H7 strain Rafaela_II

Toxin (Protein)


Gene name ataT Uniprot ID A0A891SNC1
Locus tag XF37_RS02030 Protein ID WP_000342448.1
Coordinates 418028..418555 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag XF37_RS02025 Protein ID WP_001277108.1
Coordinates 417755..418021 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
XF37_RS02005 (413413) 413413..414081 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
XF37_RS02010 (414074) 414074..415132 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
XF37_RS02015 (415377) 415377..416231 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
XF37_RS02020 (416502) 416502..417605 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
XF37_RS02025 (417755) 417755..418021 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
XF37_RS02030 (418028) 418028..418555 + 528 WP_000342448.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
XF37_RS02035 (418552) 418552..418935 - 384 WP_000778769.1 aspartate 1-decarboxylase autocleavage activator PanM -
XF37_RS02040 (419359) 419359..420468 + 1110 WP_001301528.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
XF37_RS02045 (420516) 420516..421442 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
XF37_RS02050 (421439) 421439..422716 + 1278 WP_000803797.1 branched chain amino acid ABC transporter permease LivM -
XF37_RS02055 (422713) 422713..423480 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19692.68 Da        Isoelectric Point: 7.3232

>T204767 WP_000342448.1 NZ_CP076230:418028-418555 [Escherichia coli O157:H7]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T204767 NZ_CP101245:742828-743124 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATACCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT204767 WP_001277108.1 NZ_CP076230:417755-418021 [Escherichia coli O157:H7]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT204767 NZ_CP101245:743126-743521 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6GTS

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References