Detailed information of TA system TA204698

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4175049..4175623
• Replicon: chromosome
• Accession: NZ_CP076136
• Organism: Bradyrhizobium sediminis strain S2-11-4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KMZ93_RS19965
• Protein ID: WP_215603000.1
• Coordinates: 4175258..4175623 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KMZ93_RS19960
• Protein ID: WP_215602999.1
• Coordinates: 4175049..4175264 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KMZ93_RS19935 (KMZ93_19935)	4170578..4171429	-	852	WP_215602994.1	DUF1194 domain-containing protein	-
KMZ93_RS19940 (KMZ93_19940)	4171759..4172064	+	306	WP_215602995.1	ATP-dependent Clp protease adapter ClpS	-
KMZ93_RS19945 (KMZ93_19945)	4172143..4172910	+	768	WP_215602996.1	hypothetical protein	-
KMZ93_RS19950 (KMZ93_19950)	4172942..4173673	+	732	WP_215602997.1	hypothetical protein	-
KMZ93_RS19955 (KMZ93_19955)	4173670..4174854	-	1185	WP_215602998.1	adenylate/guanylate cyclase domain-containing protein	-
KMZ93_RS19960 (KMZ93_19960)	4175049..4175264	+	216	WP_215602999.1	ribbon-helix-helix protein, CopG family	Antitoxin
KMZ93_RS19965 (KMZ93_19965)	4175258..4175623	+	366	WP_215603000.1	type II toxin-antitoxin system VapC family toxin	Toxin
KMZ93_RS19970 (KMZ93_19970)	4175620..4176708	-	1089	WP_215603001.1	chorismate synthase	-
KMZ93_RS19975 (KMZ93_19975)	4176836..4177435	-	600	WP_215603002.1	histidine phosphatase family protein	-
KMZ93_RS19980 (KMZ93_19980)	4177449..4178279	-	831	WP_215603003.1	enoyl-ACP reductase FabI	-
KMZ93_RS19985 (KMZ93_19985)	4178479..4178967	+	489	WP_215603004.1	RNA polymerase sigma factor	-
KMZ93_RS19990 (KMZ93_19990)	4179035..4179805	+	771	WP_215603005.1	anti-sigma factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-103)

Antitoxin

• Ribbon-helix-helix protein, CopG (10-39)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13688.85 Da
• Isoelectric Point: 6.7272

>T204698 WP_215603000.1 NZ_CP076136:4175258-4175623 [Bradyrhizobium sediminis]
MVKALFDTNILVDYLNAVPQARTELRRYTDKAVSIITWMEVMVGATDDVEETTRAFLSTFDVIALDEAIAERAVSLRRNH
HVKLPDAIIWATAQVHAMLLVTRKTKDFTAGDPGVRMPYRL

Nucleotide

• Length: 366 bp

>T204698 NZ_CP101222:c304919-304545 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8164.30 Da
• Isoelectric Point: 10.4652

>AT204698 WP_215602999.1 NZ_CP076136:4175049-4175264 [Bradyrhizobium sediminis]
MRTLVDLGNSQIQALDELSKKEKRSRASLIRQAIDDYLGKRRDKQAGDAFGLWGKRKVDGLAYQEKVRGEW

Nucleotide

• Length: 216 bp

>AT204698 NZ_CP101222:c305377-305009 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA