Detailed information of TA system TA204430

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 53519..54144
• Replicon: plasmid pS19_651-a
• Accession: NZ_CP075853
• Organism: Escherichia coli strain S19-651

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JFY28_RS25465
• Protein ID: WP_000911333.1
• Coordinates: 53746..54144 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: JFY28_RS25460
• Protein ID: WP_000450520.1
• Coordinates: 53519..53746 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFY28_RS25460 (53519)	53519..53746	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
JFY28_RS25465 (53746)	53746..54144	+	399	WP_000911333.1	tRNA(fMet)-specific endonuclease VapC	Toxin
JFY28_RS25470 (54153)	54153..56306	-	2154	WP_032330953.1	type IV conjugative transfer system coupling protein TraD	-
JFY28_RS25475 (56559)	56559..57269	-	711	WP_231793510.1	conjugal transfer complement resistance protein TraT	-
JFY28_RS25480 (57304)	57304..57813	-	510	WP_000628105.1	conjugal transfer entry exclusion protein TraS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3'')-Ib / aph(6)-Id / sitABCD	iutA / iucD / iucC / iucB / iucA	1..117341	117341

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14919.19 Da
• Isoelectric Point: 7.8605

>T204430 WP_000911333.1 NZ_CP075853:53746-54144 [Escherichia coli]
MLKFMLDTNICIFTMKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T204430 NZ_CP100724:c4561289-4560978 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGAGGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT204430 WP_000450520.1 NZ_CP075853:53519-53746 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT204430 NZ_CP100724:c4560977-4560687 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Antitoxin

• AlphaFold DB: V0VCB2