Detailed information of TA system TA204290

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2908391..2908993
• Replicon: chromosome
• Accession: NZ_CP075716
• Organism: Escherichia coli strain CPww7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: KKZ15_RS14230
• Protein ID: WP_000897305.1
• Coordinates: 2908682..2908993 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KKZ15_RS14225
• Protein ID: WP_000356397.1
• Coordinates: 2908391..2908681 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KKZ15_RS14200 (2904317)	2904317..2905219	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
KKZ15_RS14205 (2905216)	2905216..2905851	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
KKZ15_RS14210 (2905848)	2905848..2906777	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
KKZ15_RS14215 (2907107)	2907107..2907349	-	243	WP_001086388.1	protein YiiF	-
KKZ15_RS14220 (2907568)	2907568..2907786	-	219	WP_001295676.1	CopG family transcriptional regulator	-
KKZ15_RS14225 (2908391)	2908391..2908681	-	291	WP_000356397.1	NadS family protein	Antitoxin
KKZ15_RS14230 (2908682)	2908682..2908993	-	312	WP_000897305.1	hypothetical protein	Toxin
KKZ15_RS14235 (2909222)	2909222..2910130	+	909	WP_001162704.1	alpha/beta hydrolase	-
KKZ15_RS14240 (2910194)	2910194..2911135	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
KKZ15_RS14245 (2911180)	2911180..2911617	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
KKZ15_RS14250 (2911614)	2911614..2912486	-	873	WP_000920762.1	virulence factor BrkB family protein	-
KKZ15_RS14255 (2912480)	2912480..2913079	-	600	WP_001315111.1	glucose-1-phosphatase	-
KKZ15_RS14260 (2913178)	2913178..2913963	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T204290 WP_000897305.1 NZ_CP075716:c2908993-2908682 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T204290 NZ_CP100693:853221-853619 [Salmonella enterica subsp. enterica serovar London]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCCGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAGTTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT204290 WP_000356397.1 NZ_CP075716:c2908681-2908391 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT204290 NZ_CP100693:852994-853221 [Salmonella enterica subsp. enterica serovar London]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTCTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7