Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3366310..3367109 Replicon chromosome
Accession NZ_CP075697
Organism Escherichia coli strain STEC2018-607

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag JFX52_RS16380 Protein ID WP_000347267.1
Coordinates 3366310..3366774 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag JFX52_RS16385 Protein ID WP_001307405.1
Coordinates 3366774..3367109 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX52_RS16350 (3361311) 3361311..3361745 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
JFX52_RS16355 (3361763) 3361763..3362641 - 879 WP_001298758.1 PTS N-acetylgalactosamine transporter subunit IID -
JFX52_RS16360 (3362631) 3362631..3363410 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
JFX52_RS16365 (3363421) 3363421..3363894 - 474 WP_198777205.1 PTS N-acetylgalactosamine transporter subunit IIB -
JFX52_RS16370 (3363917) 3363917..3365197 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
JFX52_RS16375 (3365446) 3365446..3366255 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JFX52_RS16380 (3366310) 3366310..3366774 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JFX52_RS16385 (3366774) 3366774..3367109 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JFX52_RS16390 (3367258) 3367258..3368829 - 1572 WP_062850947.1 galactarate dehydratase -
JFX52_RS16395 (3369204) 3369204..3370538 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
JFX52_RS16400 (3370554) 3370554..3371324 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T204248 WP_000347267.1 NZ_CP075697:c3366774-3366310 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T204248 NZ_CP100682:c4451384-4451073 [Salmonella enterica subsp. enterica serovar Goldcoast]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT204248 WP_001307405.1 NZ_CP075697:c3367109-3366774 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT204248 NZ_CP100682:c4451072-4450782 [Salmonella enterica subsp. enterica serovar Goldcoast]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGGTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References