Detailed information of TA system TA203816

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 2955806..2956404
• Replicon: chromosome
• Accession: NZ_CP075487
• Organism: Escherichia coli strain NT1F34

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A8T6PRV3
• Locus tag: IGI26_RS14860
• Protein ID: WP_053291281.1
• Coordinates: 2955806..2956183 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: L4J1C0
• Locus tag: IGI26_RS14865
• Protein ID: WP_001603498.1
• Coordinates: 2956183..2956404 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IGI26_RS14840 (2951232)	2951232..2952773	+	1542	WP_069661456.1	IS21-like element ISEc12 family transposase	-
IGI26_RS14845 (2952788)	2952788..2953534	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
IGI26_RS14850 (2954033)	2954033..2955295	+	1263	WP_096263470.1	integrase arm-type DNA-binding domain-containing protein	-
IGI26_RS14855 (2955481)	2955481..2955696	+	216	Protein_2798	transposase	-
IGI26_RS14860 (2955806)	2955806..2956183	-	378	WP_053291281.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
IGI26_RS14865 (2956183)	2956183..2956404	-	222	WP_001603498.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
IGI26_RS14870 (2956589)	2956589..2957599	-	1011	WP_193119655.1	fimbrial protein	-
IGI26_RS14875 (2957610)	2957610..2960096	-	2487	WP_111970038.1	fimbrial biogenesis outer membrane usher protein	-
IGI26_RS14880 (2960114)	2960114..2960794	-	681	WP_053270475.1	fimbrial assembly chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	mdf(A)	msbA	2886858..3029446	142588

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13487.28 Da
• Isoelectric Point: 6.2257

>T203816 WP_053291281.1 NZ_CP075487:c2956183-2955806 [Escherichia coli]
MRHISPEELIAIHDANISRYGGLPGMPDSGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVYDSPELAELTVGAATGEVSVSTVVATLRRLYGTA

Nucleotide

• Length: 378 bp

>T203816 NZ_CP100351:1529331-1529585 [Desulfomicrobium sp. ZS1]
ATGAAACGCAAACACGAATCCACTCTTCGCAGGATTTTTGACCATCCGACATCTGGCGGGATCAAATGGGCAGATATCGA
GGCCTTGTTTGCGGAGCTGGGAGCGGAAATAGCGGAGCGTGAAGGTTCGCGCATCAGCGTGTTTCTTTTTGGAGAGATCA
GAATCTTCCATCGCCCGCATCCTTCGCCGGACACGGACAAGGGCGCGGTGGCAAGCATCAGGAAGTGGCTCGAAACGAAT
GGGGTGAAGCCATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8190.24 Da
• Isoelectric Point: 6.3159

>AT203816 WP_001603498.1 NZ_CP075487:c2956404-2956183 [Escherichia coli]
MQSINFRTARSNLTEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDTTNKKLVNR

Nucleotide

• Length: 222 bp

>AT203816 NZ_CP100351:1529582-1529920 [Desulfomicrobium sp. ZS1]
ATGAAGAACGTACTTGATTTTCCCGGCGGTTACTCTGCCGTCATTGGCTACGATTCCGAGCTGGAAATGTTCCGGGGAGA
ATTTGTCGGCTTGAATGGCGGAGCCGACTTCTACGCCACGGACCTGGAAGGACTCAAGCGAGAAGGCGCGTTGTCGCTCA
AGGTCTTTTTGGACGAATGCACCGCACGTGGCATCGAGCCCAAAAAGGCAAAAGGAAAATTCGCCCTGCGCCTGGATCAG
GACATCTACCGCCAGGCCACTATCGCCGCCGCCGCCAGCGGAAAAAGCCTGAACCAGTTCATCACCGAAGCCGTGCGCGA
AGCCGTGGCCCGCGCATGA

Structures

Antitoxin

• AlphaFold DB: L4J1C0