Detailed information of TA system TA203814

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2391197..2391835
• Replicon: chromosome
• Accession: NZ_CP075487
• Organism: Escherichia coli strain NT1F34

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: IGI26_RS12120
• Protein ID: WP_000813794.1
• Coordinates: 2391659..2391835 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: IGI26_RS12115
• Protein ID: WP_001270286.1
• Coordinates: 2391197..2391613 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IGI26_RS12095 (2386349)	2386349..2387290	-	942	WP_001251313.1	ABC transporter permease	-
IGI26_RS12100 (2387291)	2387291..2388304	-	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
IGI26_RS12105 (2388322)	2388322..2389467	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
IGI26_RS12110 (2389712)	2389712..2391118	-	1407	WP_000760663.1	PLP-dependent aminotransferase family protein	-
IGI26_RS12115 (2391197)	2391197..2391613	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
IGI26_RS12120 (2391659)	2391659..2391835	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
IGI26_RS12125 (2392057)	2392057..2392287	+	231	WP_000494244.1	YncJ family protein	-
IGI26_RS12130 (2392379)	2392379..2394340	-	1962	WP_001442195.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
IGI26_RS12135 (2394413)	2394413..2394949	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
IGI26_RS12140 (2395041)	2395041..2396231	+	1191	WP_231707766.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2396271..2397419	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T203814 WP_000813794.1 NZ_CP075487:c2391835-2391659 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T203814 NZ_CP100351:c542883-542539 [Desulfomicrobium sp. ZS1]
TTGTATCCAAATAGATACAATCTATTCATAAGCACCTTTCATCAGACCGATGTCTTCTCTTGCTGGCTCAAAAATATCAA
GGACATCAAAACCAGAGCAAGAATAGTTGCGCGCATCAGGTCTGCGGAACATGGCAATCTTGGTGATTGCACTCCAGTCG
GGGAAGGTGTGTCAGAAATGCGCCTGCACTTCGGGCCGGGTTACCGACTCTATTTCTGGCAAGAAGGTGGTCAGGTTTAC
TGGCTGCTTGCCGGGGGCGAAAAAAGCACACAGAAGCGGGATATTGAGCGGTCAAAAAAACTGCGATATGAAATTGAGGA
GATAACTCATGGTAAAAATAAGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT203814 WP_001270286.1 NZ_CP075487:c2391613-2391197 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT203814 NZ_CP100351:c542555-542262 [Desulfomicrobium sp. ZS1]
ATGGTAAAAATAAGTAAGTTTGACGCAGTGGATTATCTCGACAGCGAAGAGATGATGGCTGAATACCTTGCTGCGGCCGT
AGAAGATGATAATCCTGATGTGTTCCTCGCTGCGCTTGCTGACGTGGCAAAGGCTCGTGGCATGGCCCAACTTGCCAAAG
ACACAGGACTTGGACGTGAAAGCCTGTACAAAACCCTAGCACCTGGCGCAAAACCACGGTTTGAGACCATCATGAAAATT
ACAAAAGCACTTGGAGTTCCACTTTCTCCAAAAGCTGTAATAGCTCATACGTAA

Structures

Toxin

• PDB: 6HPB