Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3609078..3609619 Replicon chromosome
Accession NZ_CP075480
Organism Escherichia coli strain NT1N31

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag IGI30_RS17400 Protein ID WP_000615976.1
Coordinates 3609341..3609619 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag IGI30_RS17395 Protein ID WP_000729704.1
Coordinates 3609078..3609338 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI30_RS17375 (3604753) 3604753..3605538 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
IGI30_RS17380 (3605510) 3605510..3607222 + 1713 Protein_3419 flagellar biosynthesis protein FlhA -
IGI30_RS17385 (3607446) 3607446..3607943 - 498 WP_193107710.1 REP-associated tyrosine transposase RayT -
IGI30_RS17390 (3608119) 3608119..3608892 - 774 WP_015675129.1 C40 family peptidase -
IGI30_RS17395 (3609078) 3609078..3609338 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
IGI30_RS17400 (3609341) 3609341..3609619 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
IGI30_RS17405 (3609775) 3609775..3610515 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
IGI30_RS17410 (3610486) 3610486..3611253 - 768 WP_000333380.1 class II glutamine amidotransferase -
IGI30_RS17415 (3611459) 3611459..3612037 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T203764 WP_000615976.1 NZ_CP075480:3609341-3609619 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T203764 NZ_CP100326:565419-565679 [Streptococcus suis]
ATGTATCATTTAGAGTATTCTAAAAAGGCTCAAAAGCAAATTAAGAAATTAGATAGGCAAATTCAGAGGTTGTTGTTTGC
TTGGATTGATAAACACTTAGAAGGGACTGATAATCCACGCGCCAATGGTAAAGGTTTGACAGGCAATCATGCTAACGAGT
GGCGTTATCGTATCGGAGATTACCGACTGATTTGCGATATTCAAGATGATAAGTTGGTTATTTTAGCACTTGAGTTTGGA
CATCGTAAAGATGTCTATTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT203764 WP_000729704.1 NZ_CP075480:3609078-3609338 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT203764 NZ_CP100326:565196-565417 [Streptococcus suis]
ATGAGTACAGTGACAATCAGATTAAATCAAGAAGAAGAAGTATTCTTTAAAAGCTATGCACAATTAACAGGTCAAAGTCT
TTCTAGCCTTTTTAAGAAAGCTTTGGAGCGTGATATTGAAGATGAGTATGATTTAAAAATCTATCATCAAGCTTATGATG
AGTACAAAGCAGATCCAGAAACAATTAGTCATGCTGATTTTAAGAAAGAGCTAGGATTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References