Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 641064..641791 Replicon chromosome
Accession NZ_CP075480
Organism Escherichia coli strain NT1N31

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag IGI30_RS03120 Protein ID WP_000550189.1
Coordinates 641064..641378 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag IGI30_RS03125 Protein ID WP_000560266.1
Coordinates 641375..641791 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI30_RS03100 (637231) 637231..638217 - 987 WP_193107601.1 Gfo/Idh/MocA family oxidoreductase -
IGI30_RS03105 (638296) 638296..638979 - 684 WP_053295377.1 vancomycin high temperature exclusion protein -
IGI30_RS03110 (639056) 639056..639559 - 504 WP_001295542.1 M48 family metallopeptidase -
IGI30_RS03115 (639644) 639644..640780 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
IGI30_RS03120 (641064) 641064..641378 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
IGI30_RS03125 (641375) 641375..641791 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
IGI30_RS03130 (641836) 641836..643854 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
IGI30_RS03135 (644280) 644280..646631 - 2352 WP_053295378.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T203744 WP_000550189.1 NZ_CP075480:641064-641378 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T203744 NZ_CP100308:c4613882-4613541 [Klebsiella pneumoniae]
ATGAATACTTTACCTGCAATAAACCAGCGGGCTGTTCAGACATGTTTATCACCCGTGGTTGTCTGGCAAATGCTGCTTGC
TCGACTACTTGAACAGCATTATGGCCTGACCTTAAATGACACGCCGTTCAGCGATGAGGCTGTTATTAAAAGACATATCG
AAGCTGGTATCACGCTTGTCGATGCCGTTAATTTTCTGCTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAGGGGATTC
GACTGGCAAGAACAGTCCCCATATCTGCGGGCAGTCGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGAAAAGAAA
TCGTATTAGCGCAGCACGGTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT203744 WP_000560266.1 NZ_CP075480:641375-641791 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT203744 NZ_CP100308:c4614267-4613917 [Klebsiella pneumoniae]
ATGTCGAATAAGACACTGACAGTGAATGATGATACTGCTGAGCCCTGGTGGGGTTTGAGTCGTAACGTCATCCCTTGTTT
TGGCGCACGTCTGGTTCAGGAAGGTAATCGCCTGCATTACCTGGCTGGCCGCGCCAGCTTAACAGGCCAGATCCATGAAG
CGGATTTACTCCATCTTGACCAGGCGTTCCCGGTACTGCTGAAGCAGATGGAGCTCATGCTTACCTGCGGCGAACTGAAT
CCACGACACCAGCACTGCGTCACGCTTTATGCGAAGGGGCTAACCTGCGAAGCTGATACGCTGGGCTCCTGCGGTTACGT
TTATATCGCCATTTACCCCACACAACGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References