Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1365278..1365903 Replicon chromosome
Accession NZ_CP075477
Organism Escherichia coli strain NT1W25

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag IGI33_RS06625 Protein ID WP_000911329.1
Coordinates 1365505..1365903 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag IGI33_RS06620 Protein ID WP_000450524.1
Coordinates 1365278..1365505 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI33_RS06595 (1361080) 1361080..1361550 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
IGI33_RS06600 (1361550) 1361550..1362122 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
IGI33_RS06605 (1362268) 1362268..1363146 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
IGI33_RS06610 (1363163) 1363163..1364197 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
IGI33_RS06615 (1364410) 1364410..1365123 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
IGI33_RS06620 (1365278) 1365278..1365505 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
IGI33_RS06625 (1365505) 1365505..1365903 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
IGI33_RS06630 (1366050) 1366050..1366913 + 864 WP_001267498.1 neutral zinc metallopeptidase -
IGI33_RS06635 (1366928) 1366928..1368943 + 2016 WP_000829393.1 tRNA cytosine(34) acetyltransferase TmcA -
IGI33_RS06640 (1369017) 1369017..1369715 + 699 WP_000679812.1 esterase -
IGI33_RS06645 (1369825) 1369825..1370025 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T203721 WP_000911329.1 NZ_CP075477:1365505-1365903 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T203721 NZ_CP100296:2902131-2902313 [Burkholderia glumae]
ATGAACAGCGCGAACCTTATCCGAACCCTGAAAGCCGACGGCTGGTATCTCGTCAATACCGTCGGCTCGCATCACCAGTT
CAAGCATGCGACCAAGCCCGGTAAGGTGACGGTGCCTCACCCGAAGAAAGACCTTCCCCTAGGCACCGTGCGCAGCATCC
TGAAACAAGCCGGTCTGAAATGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT203721 WP_000450524.1 NZ_CP075477:1365278-1365505 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT203721 NZ_CP100296:2902340-2902735 [Burkholderia glumae]
ATGCTCTATCCGCTCTATGTCCACGTCGGCGACGACAAGCACGCACATGGCGTCACTTTTCCCGACTTCCCCGGCTGCCA
CGCTGCGGCCGACTCCTGGGATGAACTGCCCGCAGCCGTGCAGGAGGCCGCCCAGGCCCATTTCGAGGGCGACGACAGCC
CGATTCCCGAGCCTTCCGCGCTCGAAGATTTGACGCGCGATCCGGAATACACCGGCGGCGTCTGGATGATCTTCGATATC
GACCTGTCGAAGATCAACACGAAAGCCGTGCGCTTCAACGTCAGCATGTCCGAGCGGCTGCTCCAGAAGATCGACGCCAA
GGCGCGCGCGCGCAAACTCTCGCGATCGGCCTTCCTTGCGATTGCGGCAGAACACGAAATGGAAGCTGACGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References