Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1891978..1892809 Replicon chromosome
Accession NZ_CP075469
Organism Escherichia coli strain NT1W22

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag IGI32_RS09155 Protein ID WP_000854814.1
Coordinates 1891978..1892352 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B3Y195
Locus tag IGI32_RS09160 Protein ID WP_001285585.1
Coordinates 1892441..1892809 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI32_RS09115 (1887374) 1887374..1888540 + 1167 WP_016247321.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
IGI32_RS09120 (1888659) 1888659..1889132 + 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
IGI32_RS09125 (1889330) 1889330..1890388 + 1059 WP_064670363.1 FUSC family protein -
IGI32_RS09130 (1890560) 1890560..1890889 + 330 WP_000450409.1 DUF496 family protein -
IGI32_RS09135 (1890990) 1890990..1891124 - 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
IGI32_RS09140 (1891244) 1891244..1891372 + 129 Protein_1804 transposase domain-containing protein -
IGI32_RS09145 (1891661) 1891661..1891741 - 81 Protein_1805 hypothetical protein -
IGI32_RS09150 (1891787) 1891787..1891981 - 195 WP_000988600.1 DUF5983 family protein -
IGI32_RS09155 (1891978) 1891978..1892352 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
IGI32_RS09160 (1892441) 1892441..1892809 - 369 WP_001285585.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
IGI32_RS09165 (1892883) 1892883..1893104 - 222 WP_000692323.1 DUF987 domain-containing protein -
IGI32_RS09170 (1893167) 1893167..1893643 - 477 WP_001186773.1 RadC family protein -
IGI32_RS09175 (1893659) 1893659..1894138 - 480 WP_000860076.1 antirestriction protein -
IGI32_RS09180 (1894220) 1894220..1895041 - 822 WP_064670364.1 DUF932 domain-containing protein -
IGI32_RS09185 (1895262) 1895262..1895672 - 411 WP_000846713.1 hypothetical protein -
IGI32_RS09190 (1895688) 1895688..1896371 - 684 WP_000775500.1 hypothetical protein -
IGI32_RS09195 (1896507) 1896507..1897577 - 1071 WP_000102643.1 patatin-like phospholipase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - wza / gmd / wcaG / manB / wcaJ / galF / wbtL / gnd / ugd 1790299..1937536 147237


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T203650 WP_000854814.1 NZ_CP075469:c1892352-1891978 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T203650 NZ_CP100220:c112242-111841 [Burkholderia glumae]
ATGCCGCGCTTCATGCTCGACACCAATATGTGCATCTACCTGATGAAGAACCAGCCTGAGCAGGTAGCAAAGCGTTTCGC
GGAGTGCTTCGTCGGTGACGTGGTGATGTCCGCTATCACATATGCCGAGCTTGAATACGGTGTCGCGGTTTCTGACAACC
GGGCGAAAGAGCGGCGGAACCTCGCTGCGTTGATTGAGGACATTCTGGTTGCTCCGTTTGATGCTGCTGCGGCTGTAGCC
TATGGTCCGATTCGCGAGGCTACACGGGAGCGCAAGAAGGACCACCTAGACAAGTTGATCGCCGCCCATGCAGTTGCGCT
TGACGTGGTGCTTGTCACAAACAACGCGAAGGACTTCGCAAGCTATCCGGGTATCAAGCTCGACAACTGGCTGAATGACT
GA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13593.48 Da        Isoelectric Point: 6.3139

>AT203650 WP_001285585.1 NZ_CP075469:c1892809-1892441 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT203650 NZ_CP100220:c112478-112242 [Burkholderia glumae]
ATGCAGACAACCCGAGTTTTCAGGAACGGCAACTCTCAGGCCGTTCGCATCCCCGCCGATCTTGCCTATGAGCGCAGCGA
CGTTGAGCTTGAAATCGAGCGCGTGGGCGACGAAATCCGCATCCGACCGGCTCGACGTCCCTTGACGGGGGTACTCAAGA
AATTTGCCAAATTCAGCCAAGATTTCATGGCCGAGGGGCGCGGCGAGCAGGAACAAGCTGAACGTGAGGGTATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LW60

References