Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 893293..893947 Replicon chromosome
Accession NZ_CP075469
Organism Escherichia coli strain NT1W22

Toxin (Protein)


Gene name cptA Uniprot ID -
Locus tag IGI32_RS04395 Protein ID WP_151140359.1
Coordinates 893540..893947 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag IGI32_RS04390 Protein ID WP_000354046.1
Coordinates 893293..893559 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI32_RS04370 (889262) 889262..890695 - 1434 WP_001514525.1 6-phospho-beta-glucosidase BglA -
IGI32_RS04375 (890740) 890740..891051 + 312 WP_001514524.1 N(4)-acetylcytidine aminohydrolase -
IGI32_RS04380 (891215) 891215..891874 + 660 WP_000250274.1 hemolysin III family protein -
IGI32_RS04385 (892070) 892070..893050 - 981 WP_001514522.1 tRNA-modifying protein YgfZ -
IGI32_RS04390 (893293) 893293..893559 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
IGI32_RS04395 (893540) 893540..893947 + 408 WP_151140359.1 protein YgfX Toxin
IGI32_RS04400 (893987) 893987..894508 - 522 WP_001055874.1 flavodoxin FldB -
IGI32_RS04405 (894620) 894620..895516 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
IGI32_RS04410 (895541) 895541..896251 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IGI32_RS04415 (896257) 896257..897990 + 1734 WP_000813201.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16032.95 Da        Isoelectric Point: 11.2506

>T203648 WP_151140359.1 NZ_CP075469:893540-893947 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRIDARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T203648 NZ_CP100217:c131792-131490 [Burkholderia glumae]
ATGCGGGGTAAGGCGCGGGTGGTGCGGCTGACGCCCTTGGCCGAGGCCGACCTCGAGGACATCTGGGCTTACATCTTCGA
GCAGTGGTCACTCGCGCAGGCCGAGCAATACGTCGGCGAGATCGTCGCTGCCTTCAACCGACTGGCGCGCGGCGAATGGG
TCGGGGCCGTGCGGGGCGAACCTTACCGGCGCTATCTGGTCGGCTTCCATGCGGTGTTTTATCGAGAGACCACCGACGCC
TTGGATGTCGCCTGTGTGCGGCATCAGCGTATGGACGTGGGTTCGCACCTGTCGCAGTATTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT203648 WP_000354046.1 NZ_CP075469:893293-893559 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT203648 NZ_CP100217:c132027-131782 [Burkholderia glumae]
ATGCCGCGGAACACATCGATCTCGCTGGGGGATCACCTGACTCAGTTTGTCGACACACAGGTCGCATCCGGCCGCTATGG
CTCGGCCAGCGACGTGGTGCGCGCCGGGCTTCGCCTGCTCGAGACGCAGGAAACCGAACTGAAGGCGCTGAAGGAGGCGT
TGCGCGCCAGTGAGGCGTCCGGCGCGCCCGCGGCGTTTGACGGCCAAGCGTTCCTCGACCGCATGCGTGCGAAGCATGCG
GGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References