Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3637873..3638493 Replicon chromosome
Accession NZ_CP075345
Organism Enterobacter kobei strain S18_CRE18

Toxin (Protein)


Gene name Hha Uniprot ID A0A2W0G488
Locus tag KJE18_RS17410 Protein ID WP_014882802.1
Coordinates 3638275..3638493 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag KJE18_RS17405 Protein ID WP_008499288.1
Coordinates 3637873..3638247 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJE18_RS17395 (KJE18_17355) 3632999..3634192 + 1194 WP_014882804.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KJE18_RS17400 (KJE18_17360) 3634215..3637361 + 3147 WP_014882803.1 multidrug efflux RND transporter permease subunit AcrB -
KJE18_RS17405 (KJE18_17365) 3637873..3638247 + 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
KJE18_RS17410 (KJE18_17370) 3638275..3638493 + 219 WP_014882802.1 HHA domain-containing protein Toxin
KJE18_RS17415 (KJE18_17375) 3638703..3639254 + 552 WP_014882801.1 maltose O-acetyltransferase -
KJE18_RS17420 (KJE18_17380) 3639373..3639840 + 468 WP_014882800.1 YlaC family protein -
KJE18_RS17425 (KJE18_17385) 3639812..3641272 - 1461 WP_014882799.1 PLP-dependent aminotransferase family protein -
KJE18_RS17430 (KJE18_17390) 3641374..3642084 + 711 WP_038421234.1 GNAT family protein -
KJE18_RS17435 (KJE18_17395) 3642081..3642221 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
KJE18_RS17440 (KJE18_17400) 3642224..3642484 - 261 WP_010428154.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T203502 WP_014882802.1 NZ_CP075345:3638275-3638493 [Enterobacter kobei]
MSEKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T203502 NZ_CP100106:845683-846093 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGACGGATCCATGCCCGTCGCGGGGAAATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT203502 WP_008499288.1 NZ_CP075345:3637873-3638247 [Enterobacter kobei]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT203502 NZ_CP100106:845436-845702 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2W0G488

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References