Detailed information of TA system TA203362

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5572594..5573197
• Replicon: chromosome
• Accession: NZ_CP075183
• Organism: Hydrogenophaga taeniospiralis strain LT2

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: KI616_RS26475
• Protein ID: WP_226464144.1
• Coordinates: 5572919..5573197 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KI616_RS26470
• Protein ID: WP_226464143.1
• Coordinates: 5572594..5572896 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI616_RS26445 (KI616_26370)	5568336..5568686	+	351	WP_226464140.1	phage holin family protein	-
KI616_RS26450 (KI616_26375)	5568932..5570479	-	1548	WP_226467144.1	M48 family metalloprotease	-
KI616_RS26455 (KI616_26380)	5570725..5571231	+	507	WP_226464141.1	cyclic pyranopterin monophosphate synthase MoaC	-
KI616_RS26460 (KI616_26385)	5571339..5572340	+	1002	WP_226464142.1	GSU2403 family nucleotidyltransferase fold protein	-
KI616_RS26465	5572389..5572520	+	132	Protein_5215	cyclic pyranopterin monophosphate synthase MoaC	-
KI616_RS26470 (KI616_26395)	5572594..5572896	-	303	WP_226464143.1	HigA family addiction module antitoxin	Antitoxin
KI616_RS26475 (KI616_26400)	5572919..5573197	-	279	WP_226464144.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KI616_RS26480 (KI616_26405)	5573299..5573508	+	210	Protein_5218	hypothetical protein	-
KI616_RS26485 (KI616_26410)	5573560..5574336	-	777	WP_226464145.1	TfpX/TfpZ family type IV pilin accessory protein	-
KI616_RS26490 (KI616_26415)	5574390..5574815	-	426	WP_226467145.1	pilin	-
KI616_RS26495	5574798..5574935	-	138	Protein_5221	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
KI616_RS26500 (KI616_26420)	5575165..5575713	-	549	WP_226464146.1	pilin	-
KI616_RS26505 (KI616_26425)	5575901..5576704	-	804	WP_226464147.1	3',5'-cyclic-nucleotide phosphodiesterase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-66)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10421.82 Da
• Isoelectric Point: 7.8871

>T203362 WP_226464144.1 NZ_CP075183:c5573197-5572919 [Hydrogenophaga taeniospiralis]
MIQSFKCGDAQALYETGKSRRFVNFKEVAERKLTQLAAATTLEFLRSPPGNHLEALSGDRVGQHSVRINKQWRVCFVWTA
EGPADVEIVDYH

Nucleotide

• Length: 279 bp

>T203362 NZ_CP099973:c2693603-2693500 [Salmonella enterica subsp. enterica serovar Enteritidis]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11125.89 Da
• Isoelectric Point: 8.7647

>AT203362 WP_226464143.1 NZ_CP075183:c5572896-5572594 [Hydrogenophaga taeniospiralis]
MIKNGMRPVHPGEVLREDFLKPLGMSANALSRALHVPAGRVNDIVLERRGVTPDTALRLARYFGGDAQSWLNLQQMHDLK
LAERAMASRIEAEIEPMAAH

Nucleotide

• Length: 303 bp

>AT203362 NZ_CP099973:2693498-2693643 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG